中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2011年
3期
297-298
,共2页
孙艳荪%张宇光%苏本利%李昌臣
孫豔蓀%張宇光%囌本利%李昌臣
손염손%장우광%소본리%리창신
糖尿病%解偶联蛋白-2%糖尿病血管病变%多态性,单核苷酸
糖尿病%解偶聯蛋白-2%糖尿病血管病變%多態性,單覈苷痠
당뇨병%해우련단백-2%당뇨병혈관병변%다태성,단핵감산
Diabetes mellitus%Uncoupling protein 2%Diabetic angiopathies%Polymorphism,single nucleotide
目的 探讨解偶联蛋白2(UCP-2)基因8号外显子3'-末端(3'-UTR)45bp插入/缺失多态性与糖尿病并发大血管病变的关系.方法 182例糖尿病患者中并发大血管病变患者80例(A组)、无大血管病变患者102例(B组),检测两组基因组DNA,用聚合酶链反应(PCR)方法对UCP-2基因进行扩增,记录各组UCP-2等位基因及基因型频率并进行比较.结果 UCP-2基因3'-UTR 45bp插入/缺失多态性在A组和B组中基因型分布分别为(Ⅱ:6.25%、ID:18.75%、DD:75.00%)和(Ⅱ:9.80%、ID:23.53%、DD:66.67%),两组差异均无统计学意义(均P＞0.05),两组等位基因频率分别为(Ⅰ:15.63%、D:84.37%)和(Ⅰ:21.57%、D:78.43%),两组间差异均无统计学意义(均P＞0.05).结论 UCP-2基因8号外显子3'-末端(3'-UTR)45bp插入/缺失多态性与糖尿病并发大血管病变无相关性.
目的 探討解偶聯蛋白2(UCP-2)基因8號外顯子3'-末耑(3'-UTR)45bp插入/缺失多態性與糖尿病併髮大血管病變的關繫.方法 182例糖尿病患者中併髮大血管病變患者80例(A組)、無大血管病變患者102例(B組),檢測兩組基因組DNA,用聚閤酶鏈反應(PCR)方法對UCP-2基因進行擴增,記錄各組UCP-2等位基因及基因型頻率併進行比較.結果 UCP-2基因3'-UTR 45bp插入/缺失多態性在A組和B組中基因型分佈分彆為(Ⅱ:6.25%、ID:18.75%、DD:75.00%)和(Ⅱ:9.80%、ID:23.53%、DD:66.67%),兩組差異均無統計學意義(均P＞0.05),兩組等位基因頻率分彆為(Ⅰ:15.63%、D:84.37%)和(Ⅰ:21.57%、D:78.43%),兩組間差異均無統計學意義(均P＞0.05).結論 UCP-2基因8號外顯子3'-末耑(3'-UTR)45bp插入/缺失多態性與糖尿病併髮大血管病變無相關性.
목적 탐토해우련단백2(UCP-2)기인8호외현자3'-말단(3'-UTR)45bp삽입/결실다태성여당뇨병병발대혈관병변적관계.방법 182례당뇨병환자중병발대혈관병변환자80례(A조)、무대혈관병변환자102례(B조),검측량조기인조DNA,용취합매련반응(PCR)방법대UCP-2기인진행확증,기록각조UCP-2등위기인급기인형빈솔병진행비교.결과 UCP-2기인3'-UTR 45bp삽입/결실다태성재A조화B조중기인형분포분별위(Ⅱ:6.25%、ID:18.75%、DD:75.00%)화(Ⅱ:9.80%、ID:23.53%、DD:66.67%),량조차이균무통계학의의(균P＞0.05),량조등위기인빈솔분별위(Ⅰ:15.63%、D:84.37%)화(Ⅰ:21.57%、D:78.43%),량조간차이균무통계학의의(균P＞0.05).결론 UCP-2기인8호외현자3'-말단(3'-UTR)45bp삽입/결실다태성여당뇨병병발대혈관병변무상관성.
Objective To investigate the effects of uncoupling protein 2 (UCP-2) gene a 45 bp insertion/deletion (Ins/Del) polymorphism in the 3'-untranslated (3'-UTR) of its exon 8 on macroangiopathy in diabetes mellitus.Methods A total of 182 patients were selected,80 cases with macroangiopathy( A group), 102 cases without macroangiopathy(B group) ,UCP-2 gene polymorphism was confirmed by electrophoresis after PCR with 3% agarose,then compared genotype and allele gene frequency. Results The 3'-UTR Ins/Del polymorphism of UCP-2 gene in A group ( II:6. 25% 、ID: 18. 75% 、DD:75.00% ) and B group( II:9. 80% 、ID:23.53%、 DD: 66. 67% ) had no significant difference ( P ＞ 0. 05 ), and there was also no difference of alleles frequencies in two groups ( I: 15.63 %、 D: 84. 37 % )and(I:21.57% 、D:78.43%)(P ＞0.05). Conclusion No relationship of the 3'-UTR a 45bp Ina/Del polymorphism in exon 8 of the UCP-2 gene was found with macroangiopathy in diabetes mellitus.
