中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
11期
1053-1056
,共4页
郑友限%陈明春%王耿%龚彩婷%陈杰毅%林锦忠
鄭友限%陳明春%王耿%龔綵婷%陳傑毅%林錦忠
정우한%진명춘%왕경%공채정%진걸의%림금충
H1N1流感病毒%Real-time%RT-PCR%序列分析
H1N1流感病毒%Real-time%RT-PCR%序列分析
H1N1류감병독%Real-time%RT-PCR%서렬분석
H1N1 influenza virus%Real-time RT-PCR%Sequence analysis
目的 了解2009年泉州地区H1N1流感监测情况,分析泉州市H1N1流感病毒的HA和NA基因特征,探讨该病毒的遗传变异及分子特性.方法 对泉州市H1N1流感监测期间的病人咽拭子采用real-time RT-PCR方法检测病毒核酸,MDCK细胞培养进行病毒分离、鉴定,并提取其中2株代表性毒株病毒RNA;采用RT-PCR扩增病毒HA和NA基因,纯化产物进行核苷酸序列测定;用DNAStar Megalign软件进行序列分析.结果 1020份咽拭子中有200份为H1N1流感病毒核酸阳性,70份季节性流感病毒核酸阳性,其中53份为H3N2亚型,14份为H1N1亚型,3份为B型,并分离到29株甲型H1N1流感病毒株.HA基因经核苷酸序列测定显示,该毒株与北美流行株高度同源,由HA基因核苷酸序列推导的氨基酸系列与疫苗株A/Brisbane/59/2007相比,有22个位于抗原决定簇的氨基酸位点发生变异,但受体结合特异性仍为人样受体.NA基因耐药性位点分析,显示对达菲药物依然敏感.结论 2009年泉州市H1N1流感流行毒株与北美流行株高度同源,相对于疫苗代表株出现了HA蛋白抗原性的改变.
目的 瞭解2009年泉州地區H1N1流感鑑測情況,分析泉州市H1N1流感病毒的HA和NA基因特徵,探討該病毒的遺傳變異及分子特性.方法 對泉州市H1N1流感鑑測期間的病人嚥拭子採用real-time RT-PCR方法檢測病毒覈痠,MDCK細胞培養進行病毒分離、鑒定,併提取其中2株代錶性毒株病毒RNA;採用RT-PCR擴增病毒HA和NA基因,純化產物進行覈苷痠序列測定;用DNAStar Megalign軟件進行序列分析.結果 1020份嚥拭子中有200份為H1N1流感病毒覈痠暘性,70份季節性流感病毒覈痠暘性,其中53份為H3N2亞型,14份為H1N1亞型,3份為B型,併分離到29株甲型H1N1流感病毒株.HA基因經覈苷痠序列測定顯示,該毒株與北美流行株高度同源,由HA基因覈苷痠序列推導的氨基痠繫列與疫苗株A/Brisbane/59/2007相比,有22箇位于抗原決定簇的氨基痠位點髮生變異,但受體結閤特異性仍為人樣受體.NA基因耐藥性位點分析,顯示對達菲藥物依然敏感.結論 2009年泉州市H1N1流感流行毒株與北美流行株高度同源,相對于疫苗代錶株齣現瞭HA蛋白抗原性的改變.
목적 료해2009년천주지구H1N1류감감측정황,분석천주시H1N1류감병독적HA화NA기인특정,탐토해병독적유전변이급분자특성.방법 대천주시H1N1류감감측기간적병인인식자채용real-time RT-PCR방법검측병독핵산,MDCK세포배양진행병독분리、감정,병제취기중2주대표성독주병독RNA;채용RT-PCR확증병독HA화NA기인,순화산물진행핵감산서렬측정;용DNAStar Megalign연건진행서렬분석.결과 1020빈인식자중유200빈위H1N1류감병독핵산양성,70빈계절성류감병독핵산양성,기중53빈위H3N2아형,14빈위H1N1아형,3빈위B형,병분리도29주갑형H1N1류감병독주.HA기인경핵감산서렬측정현시,해독주여북미류행주고도동원,유HA기인핵감산서렬추도적안기산계렬여역묘주A/Brisbane/59/2007상비,유22개위우항원결정족적안기산위점발생변이,단수체결합특이성잉위인양수체.NA기인내약성위점분석,현시대체비약물의연민감.결론 2009년천주시H1N1류감류행독주여북미류행주고도동원,상대우역묘대표주출현료HA단백항원성적개변.
Objective To investigate the influenza H1N1 virus surveillance of 2009 in Quanzhou,and analyze the HA and NA gene of influenza H1N1 virus, explore its genetic variation and molecular characteristics. Methods During the influenza H1N1 virus surveillance in Quanzhou,specimens of throat swabs from the patients with influenza were collected, and detected by real-time RT-PCR. Viruses were isolated with MDCK cells and identified with serological test. Two influenza virus isolates were extracted, and their HA and NA genes were amplified by RT-PCR. The purified PCR products were sequenced. The data obtained were analyzed with the software DNAMAN. Results Of 1020, influenza H1N1 virus RNA was detected in 200 specimens, seasonal influenza virus RNA was detected in 70 specimens. A total of 29 influenza A H1N1 virus strains were isolated. The nucleotide homology in the HA gene was highly homologous with that of pandemic influenza virus in North America. The amino acids sequences deduced from the nucleotide sequences in HA region of the isolated strain had 22 variations compared with A/Brisbane/59/2007 vaccine strain recommend by WHO,the characteristics of α2,6 sialic acid receptor binding remained. The analysis of amino acids sequences of NA indicated that this virus possessed Oseltamivir sensitivity. Conclusion The causative influenza H1N1 strains in Quanzhou is highly homologous with that of pandemic influenza in North America, and it is antigenically and genetically different from the vaccine strain.
