CAJ | 학술논문

目的观察外源性人金属硫蛋白1E(MT1E)基因对胰腺癌SW1990细胞生长的影响.方法构建MT1E基因真核表达载体,转染SW1990细胞并获得阳性单克隆细胞.用Westernblot技术检测转染前后MT1E蛋白表达,噻唑蓝(MTT)实验检测其增殖能力的变化,流式细胞仪分析细胞周期时相分布.同时检测Cyclin D1与p53的表达.结果 MT1E融合蛋白在SW1990MT1E细胞中表达.MTT结果表明,与SW1990和SW1990MT1E空细胞比较,SW1990MT1E细胞增殖速度明显增快.SW1990MT1E细胞与SW1990和SW1990空细胞比较,G0/G1期细胞明显减少,S期明显增多,差异有统计学意义.SW1990MT1E较SW1990空的细胞Cyclin D1与p53表达明显升高.结论 MT1E能够促进SW1990增殖,可能和上调Cyclin D1促使细胞进入S期增多,通过失活p53蛋白使细胞恶性变.
목적 관찰외원성인금속류단백1E(MT1E)기인대이선암SW1990세포생장적영향.방법 구건MT1E기인진핵표체재체,전염SW1990세포병획득양성단극륭세포.용Westernblot기술검측전염전후MT1E단백표체,새서람(MTT)실험검측기증식능력적변화,류식세포의분석세포주기시상분포.동시검측Cyclin D1여p53적표체.결과 MT1E융합단백재SW1990MT1E세포중표체.MTT결과표명,여SW1990화SW1990MT1E공세포비교,SW1990MT1E세포증식속도명현증쾌.SW1990MT1E세포여SW1990화SW1990공세포비교,G0/G1기세포명현감소,S기명현증다,차이유통계학의의.SW1990MT1E교SW1990공적세포Cyclin D1여p53표체명현승고.결론 MT1E능구촉진SW1990증식,가능화상조Cyclin D1촉사세포진입S기증다,통과실활p53단백사세포악성변.
Objective To investigate the effects of MT1E gene on the cell proliferation in human pancreatic carcinoma cell line SW1990.Methods Human pancreatic carcinoma cell line SW1990 made by transfection with expressible MT1E gene, Positive monoclone was screened by G418.Western blotting assays were used to identify the expression of MT1E protein respectively. The cell proliferation was determined by MTT assay. The cell cycle was detected by flow cytometry, The apoptosis-related protein levels of Cyclin Dl and p53 determined by immunohistochemistry. Results MT1E protein were expressed in SW1990.Cells tranfected pcDNA3.1 ( - ) -MT1E compared with the SW1990 and cells tranfected pcDNA 3.1 ( - ), proliferation rate significantly enhanced, cells number of G0/G1 phase obviously decreased while cells number of S phase increased, Cyclin Dl and p53 protein levels elevated. Conclusion MT1E could promote proliferation of SW1990 cell by upregulating expression of cyclin Dl and p53 to facilitate cells into S phase.