目的 调查温州地区健康成人RET-He及网织红分群值正常值范围.方法 选取2008年8月至2010年2月温州地区1 000名健康体检者,并按性别和年龄分组.按性别分组:男506名,女494名.按年龄分组:20～40岁组341名;＞40～60岁组316名;＞60岁组343名.采集所有调查对象2 ml乙二胺四乙酸二钾(EDTA-K2)抗凝静脉血,利用Sysmex XE-2100五分类自动血细胞分析仪对1 000名健康成人外周血RET-He及网织红分群值(IRF、LFR、MFR、HFR)进行检测,确定其正常值范围.仪器测定血标本之前进行校准和验证,所有标本采集后2 h内完成检测.RET-He、RET绝对值、RET百分数为正态分布数据,采用((x)-1.96 s,(x)+1.96 s)确定正常值范围.网织红分群值(IRF、LFR、MFR、HFR)为偏态分布数据,采用百分位数(P2.5,P97.5)来确定正常值范围.结果 男性RET-He均值为(33.91±1.77)pg,正常值范围为(30.44～37.38)pg,女性均值为(33.97±1.85)pg,正常值范围为(30.34～37.60)pg,差异无统计学意义(t=-0.533,P＞0.05).男性RET绝对值均值为(0.051±0.023)×1012/L,正常值范围为(0.006～0.096)×1012/L,女性均值为(0.037±0.026)×1012/L,正常值范围为(0～0.088)×1012/L,差异有统计学意义(t=8.409,P＜0.05).男性RET百分数均值为(1.041±0.459)%,正常值范围为(0.141～1.941)%,女性均值为(0.869±0.603)%,正常值范围为(0～2.051)%,差异有统计学意义(t=5.074,P＜0.05).男性LFR、MFR、HFR、IRF正常值范围分别为0.951(0.866～0.988)、0.046(0.010～0.114)、0.003(0～0.023)、0.050(0.012～0.134),女性分别为0.096(0.880～0.991)、0.039(0.008～0.113)、0.002(0～0.016)、0.040(0.009～0.121),差异均有统计学意义(Z=-5.044、-4.793、-3.559、-5.075,P均＜0.05).20～40岁组、＞40～60岁组、＞60岁组和全年龄段RET-He均值分别为(33.38±1.49)、(34.36±1.46)、(34.12±2.21)、(33.94±1.81)pg,正常值范围分别为(30.46～36.30)、(31.50～37.22)、(29.79～38.45)、(30.39～37.49)Pg,差异有统计学意义(F=28.072,P＜0.05),且20～40岁组与＞40～60岁组、＞60岁组比较,差异均有统计学意义(q=9.970、7.791,P均＜0.05).＞60岁组LFR、MFR、HFR、IRF正常值范围分别是0.961(0.878～0.992)、0.037(0.007～0.105)、0.002(0～0.020)、0.039(0.008～0.117),与20～40岁组的0.953(0.867～0.990)、0.045(0.009～0.116)、0.003(0～0.019)、0.050(0.010～0.133)比较,差异有统计学意义(Z=-3.949、-4.236、-4.373、-4.973,P均＜0.05),与＞40～60岁组的0.951(0.855～0.989)、0.047(0.010～0.133)、0.003(0～0.020)、0.049(0.011～0.149)比较,差异亦有统计学意义(Z=-2.747、-3.275、-3.901、-4.185,P均＜0.05).RET-He测定值在不同性别组间差异无统计学意义,可以制定同一正常值范围;但RET-He在不同年龄组间差异有统计学意义,应制定不同的正常值范围.LFR、MFR、HFR、IRF在不同性别及不同年龄组间差异均有统计学意义,应按性别和年龄分组制定正常值范围.结论 本研究建立了温州地区健康成人外周血RETHe及网织红分群值正常值范围,提高了网织红细胞参数的临床应用价值.
目的 調查溫州地區健康成人RET-He及網織紅分群值正常值範圍.方法 選取2008年8月至2010年2月溫州地區1 000名健康體檢者,併按性彆和年齡分組.按性彆分組:男506名,女494名.按年齡分組:20～40歲組341名;＞40～60歲組316名;＞60歲組343名.採集所有調查對象2 ml乙二胺四乙痠二鉀(EDTA-K2)抗凝靜脈血,利用Sysmex XE-2100五分類自動血細胞分析儀對1 000名健康成人外週血RET-He及網織紅分群值(IRF、LFR、MFR、HFR)進行檢測,確定其正常值範圍.儀器測定血標本之前進行校準和驗證,所有標本採集後2 h內完成檢測.RET-He、RET絕對值、RET百分數為正態分佈數據,採用((x)-1.96 s,(x)+1.96 s)確定正常值範圍.網織紅分群值(IRF、LFR、MFR、HFR)為偏態分佈數據,採用百分位數(P2.5,P97.5)來確定正常值範圍.結果 男性RET-He均值為(33.91±1.77)pg,正常值範圍為(30.44～37.38)pg,女性均值為(33.97±1.85)pg,正常值範圍為(30.34～37.60)pg,差異無統計學意義(t=-0.533,P＞0.05).男性RET絕對值均值為(0.051±0.023)×1012/L,正常值範圍為(0.006～0.096)×1012/L,女性均值為(0.037±0.026)×1012/L,正常值範圍為(0～0.088)×1012/L,差異有統計學意義(t=8.409,P＜0.05).男性RET百分數均值為(1.041±0.459)%,正常值範圍為(0.141～1.941)%,女性均值為(0.869±0.603)%,正常值範圍為(0～2.051)%,差異有統計學意義(t=5.074,P＜0.05).男性LFR、MFR、HFR、IRF正常值範圍分彆為0.951(0.866～0.988)、0.046(0.010～0.114)、0.003(0～0.023)、0.050(0.012～0.134),女性分彆為0.096(0.880～0.991)、0.039(0.008～0.113)、0.002(0～0.016)、0.040(0.009～0.121),差異均有統計學意義(Z=-5.044、-4.793、-3.559、-5.075,P均＜0.05).20～40歲組、＞40～60歲組、＞60歲組和全年齡段RET-He均值分彆為(33.38±1.49)、(34.36±1.46)、(34.12±2.21)、(33.94±1.81)pg,正常值範圍分彆為(30.46～36.30)、(31.50～37.22)、(29.79～38.45)、(30.39～37.49)Pg,差異有統計學意義(F=28.072,P＜0.05),且20～40歲組與＞40～60歲組、＞60歲組比較,差異均有統計學意義(q=9.970、7.791,P均＜0.05).＞60歲組LFR、MFR、HFR、IRF正常值範圍分彆是0.961(0.878～0.992)、0.037(0.007～0.105)、0.002(0～0.020)、0.039(0.008～0.117),與20～40歲組的0.953(0.867～0.990)、0.045(0.009～0.116)、0.003(0～0.019)、0.050(0.010～0.133)比較,差異有統計學意義(Z=-3.949、-4.236、-4.373、-4.973,P均＜0.05),與＞40～60歲組的0.951(0.855～0.989)、0.047(0.010～0.133)、0.003(0～0.020)、0.049(0.011～0.149)比較,差異亦有統計學意義(Z=-2.747、-3.275、-3.901、-4.185,P均＜0.05).RET-He測定值在不同性彆組間差異無統計學意義,可以製定同一正常值範圍;但RET-He在不同年齡組間差異有統計學意義,應製定不同的正常值範圍.LFR、MFR、HFR、IRF在不同性彆及不同年齡組間差異均有統計學意義,應按性彆和年齡分組製定正常值範圍.結論 本研究建立瞭溫州地區健康成人外週血RETHe及網織紅分群值正常值範圍,提高瞭網織紅細胞參數的臨床應用價值.
목적 조사온주지구건강성인RET-He급망직홍분군치정상치범위.방법 선취2008년8월지2010년2월온주지구1 000명건강체검자,병안성별화년령분조.안성별분조:남506명,녀494명.안년령분조:20～40세조341명;＞40～60세조316명;＞60세조343명.채집소유조사대상2 ml을이알사을산이갑(EDTA-K2)항응정맥혈,이용Sysmex XE-2100오분류자동혈세포분석의대1 000명건강성인외주혈RET-He급망직홍분군치(IRF、LFR、MFR、HFR)진행검측,학정기정상치범위.의기측정혈표본지전진행교준화험증,소유표본채집후2 h내완성검측.RET-He、RET절대치、RET백분수위정태분포수거,채용((x)-1.96 s,(x)+1.96 s)학정정상치범위.망직홍분군치(IRF、LFR、MFR、HFR)위편태분포수거,채용백분위수(P2.5,P97.5)래학정정상치범위.결과 남성RET-He균치위(33.91±1.77)pg,정상치범위위(30.44～37.38)pg,녀성균치위(33.97±1.85)pg,정상치범위위(30.34～37.60)pg,차이무통계학의의(t=-0.533,P＞0.05).남성RET절대치균치위(0.051±0.023)×1012/L,정상치범위위(0.006～0.096)×1012/L,녀성균치위(0.037±0.026)×1012/L,정상치범위위(0～0.088)×1012/L,차이유통계학의의(t=8.409,P＜0.05).남성RET백분수균치위(1.041±0.459)%,정상치범위위(0.141～1.941)%,녀성균치위(0.869±0.603)%,정상치범위위(0～2.051)%,차이유통계학의의(t=5.074,P＜0.05).남성LFR、MFR、HFR、IRF정상치범위분별위0.951(0.866～0.988)、0.046(0.010～0.114)、0.003(0～0.023)、0.050(0.012～0.134),녀성분별위0.096(0.880～0.991)、0.039(0.008～0.113)、0.002(0～0.016)、0.040(0.009～0.121),차이균유통계학의의(Z=-5.044、-4.793、-3.559、-5.075,P균＜0.05).20～40세조、＞40～60세조、＞60세조화전년령단RET-He균치분별위(33.38±1.49)、(34.36±1.46)、(34.12±2.21)、(33.94±1.81)pg,정상치범위분별위(30.46～36.30)、(31.50～37.22)、(29.79～38.45)、(30.39～37.49)Pg,차이유통계학의의(F=28.072,P＜0.05),차20～40세조여＞40～60세조、＞60세조비교,차이균유통계학의의(q=9.970、7.791,P균＜0.05).＞60세조LFR、MFR、HFR、IRF정상치범위분별시0.961(0.878～0.992)、0.037(0.007～0.105)、0.002(0～0.020)、0.039(0.008～0.117),여20～40세조적0.953(0.867～0.990)、0.045(0.009～0.116)、0.003(0～0.019)、0.050(0.010～0.133)비교,차이유통계학의의(Z=-3.949、-4.236、-4.373、-4.973,P균＜0.05),여＞40～60세조적0.951(0.855～0.989)、0.047(0.010～0.133)、0.003(0～0.020)、0.049(0.011～0.149)비교,차이역유통계학의의(Z=-2.747、-3.275、-3.901、-4.185,P균＜0.05).RET-He측정치재불동성별조간차이무통계학의의,가이제정동일정상치범위;단RET-He재불동년령조간차이유통계학의의,응제정불동적정상치범위.LFR、MFR、HFR、IRF재불동성별급불동년령조간차이균유통계학의의,응안성별화년령분조제정정상치범위.결론 본연구건립료온주지구건강성인외주혈RETHe급망직홍분군치정상치범위,제고료망직홍세포삼수적림상응용개치.
Objective To investigate the normal ranges of RET-He and reticulocyte grouping parameters of healthy adults in Wenzhou. Methods A total of 1 000 samples from healthy adults were collected in Wenzhou from August 2008 to February 2010. There were 506 males and 494 females. The individuals were stratified based on age into three groups:20 to 40-year-old group (n = 341 ), ＞40 to 60-year-old group (n =316), and ＞60-year-old group (n =343). All blood samples were drawn in EDTA-K2 anticoagulated blood. Peripheral RET-He and reticulocyte grouping parameters ( IRF, LFR, MFR, HFR)were determined by Sysmex XE-2100 automated hematology analyzer. The instrument was calibrated and validated before testing All specimens were analyzed within 2 hours. RET-He, RET absolute value and RET percentage were normal distribution, the normal range was established by using ((x)-1.96s,(x)+ 1.96 s)method. IRF, LFR, MFR and HFR data were skewed distribution,the normal range was established by using (P2.5 ,P97.5 )percentile method. Results The mean and normal range of RET-He were (33.91 ± 1.77) pg and (30.44 - 37.38) pg in male, (33.97 ± 1.85) pg and (30.34 - 37.60) pg in female,respectively. There was no significant difference between the two groups ( t =-0.533, P＞0.05 ). The mean and normal range of RET absolute value were (0.051±0.023) × 1012/L and (0.006 -0.096)×1012/L in male,which were (0. 037 ±0. 026) × 1012/L and (0 -0. 088) × 1012/L in female, there was significant difference between the two groups ( t = 8. 409, P ＜ 0. 05 ) . The mean and normal range of RET percentage in male were(1.041 ±0.459)% and (0. 141 -1.941)% , and in female were (0.869±0.603)% and (0-2. 051 )%, which showed significant difference (t =5.074,P ＜0. 05). The normal ranges of LFR,MFR,HFR,IRF in male were 0. 951 (0.866 -0.988) ,0.046(0.010 -0.114) ,0. 003(0 -0.023) ,0. 050(0.012-0.134), and in female were 0.096(0.880 -0.991) ,0.039(0.008 -0.113) ,0.002(0-0.016) ,0.040(0.009-0.121), respectively. There were significant differences between the two groups (Z values were -5.044, -4.793, -3.559, -5.075, respectively,all P＜0.05). The means of RET-He in 20 to 40-year-old group, ＞40 to 60-year-old group, ＞ 60-year-old group and all age group were (33.38±1.49),(34.36 ±1.46), (34.12±2.21) and (33.94 +1.81) pg, respectively. The normal ranges were(30.46-36.30), (31.50 -37.22), (29.79 - 38.45) and (30.39 - 37.49) pg, which showed statistical difference( F =28. 072,P ＜0. 05). In addition, the normal range of RET-He in 20 to 40-year-old group was lower than that of ＞ 40 to 60-year-old group and ＞ 60-year-old group ( q values were 9. 970, 7. 791,respectively, P＜0. 05). The normal ranges of LFR, MFR, HFR, IRF in ＞ 60-year-old group were 0.961(0. 878 -0. 992) ,0. 037(0. 007 -0. 105 ) ,0. 002(0 -0. 020) and 0. 039(0. 008 -0. 117) ,while they were 0.953(0. 867 -0.990), 0.045(0.009 -0. 116) ,0.003(0 -0.019) ,0. 050(0. 010 -0. 133) in 20 to 40 -year-old group , and 0. 951 (0.855 -0.989) ,0.047 (0.010 - 0.133 ) ,0.003 ( 0-0.020), 0.049 (0.011-0.149)in ＞40 to 60-year-old group, which showed statistical difference (Z values were -3. 949, -4.236,-4. 373, -4. 973, - 2. 747, - 3. 275, - 3.901, - 4. 185, respectively, all P ＜ 0. 05). There was nodifference between male and female for RET-He, the same normal ranges could be used, but there was significant difference between different age groups. LFR, MFR, HFR and IRF were statistically different between different age groups and different sex groups. The normal ranges should be established by gender and age. Conclusion The normal ranges of RET-He and reticulocyte grouping parameters about healthy adults in Wenzhou are established, which plays an important role in clinical value of RET parameters.
