中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
2期
113-117
,共5页
曲梅%黄芳%张新%张代涛%刘桂荣%崔志刚%严寒秋%刘元%何战英%窦相峰%阚飙%王全意
麯梅%黃芳%張新%張代濤%劉桂榮%崔誌剛%嚴寒鞦%劉元%何戰英%竇相峰%闞飆%王全意
곡매%황방%장신%장대도%류계영%최지강%엄한추%류원%하전영%두상봉%감표%왕전의
沙门菌属%电泳,凝胶,脉冲场%细菌分型技术
沙門菌屬%電泳,凝膠,脈遲場%細菌分型技術
사문균속%전영,응효,맥충장%세균분형기술
Salmonella%Electrophoresis,gel,pulsed-field%Bacterial typing techniques
目的 分析2008-2009年北京市沙门菌流行特征及脉冲场凝胶电泳(PFGE)分子分型. 方法 对2008-2009年通过WHO全球沙门菌监测系统及北京市肠道门诊监测系统分离到的137株沙门菌进行生化鉴定、血清分型和相关流行病学分析;利用PFGE进行分子分型. 结果 北京市2008-2009年沙门菌的流行具有明显季节性,6-9月份高发,共分离菌株84株,占64.1%(84/131);患者年龄多为18～40岁,占46.1%(58/128);男性80例,女性51例,男女比例为1.57:1.137株沙门菌分属于20种血清型,其中肠炎沙门菌和鼠伤寒沙门菌为优势菌型,分别占46.7%(64/137)和17.5%(24/137).共分71种PFGE带型,其中肠炎沙门菌和鼠伤寒沙门菌都有16种PFGE型别.肠炎沙门菌的4种PFGE型别(JEGX01.CN0001、JEGX01.CN0003、JFGX01.CN0002、JEGX01.CN0019)和鼠伤寒沙门菌的JPXX01.CN0001为优势分子型别. 结论 2008-2009年北京市沙门菌的流行具有性别、年龄和季节性分布特征;PFGE分子型别较多,且存在差异明显的多个克隆系.
目的 分析2008-2009年北京市沙門菌流行特徵及脈遲場凝膠電泳(PFGE)分子分型. 方法 對2008-2009年通過WHO全毬沙門菌鑑測繫統及北京市腸道門診鑑測繫統分離到的137株沙門菌進行生化鑒定、血清分型和相關流行病學分析;利用PFGE進行分子分型. 結果 北京市2008-2009年沙門菌的流行具有明顯季節性,6-9月份高髮,共分離菌株84株,佔64.1%(84/131);患者年齡多為18～40歲,佔46.1%(58/128);男性80例,女性51例,男女比例為1.57:1.137株沙門菌分屬于20種血清型,其中腸炎沙門菌和鼠傷寒沙門菌為優勢菌型,分彆佔46.7%(64/137)和17.5%(24/137).共分71種PFGE帶型,其中腸炎沙門菌和鼠傷寒沙門菌都有16種PFGE型彆.腸炎沙門菌的4種PFGE型彆(JEGX01.CN0001、JEGX01.CN0003、JFGX01.CN0002、JEGX01.CN0019)和鼠傷寒沙門菌的JPXX01.CN0001為優勢分子型彆. 結論 2008-2009年北京市沙門菌的流行具有性彆、年齡和季節性分佈特徵;PFGE分子型彆較多,且存在差異明顯的多箇剋隆繫.
목적 분석2008-2009년북경시사문균류행특정급맥충장응효전영(PFGE)분자분형. 방법 대2008-2009년통과WHO전구사문균감측계통급북경시장도문진감측계통분리도적137주사문균진행생화감정、혈청분형화상관류행병학분석;이용PFGE진행분자분형. 결과 북경시2008-2009년사문균적류행구유명현계절성,6-9월빈고발,공분리균주84주,점64.1%(84/131);환자년령다위18～40세,점46.1%(58/128);남성80례,녀성51례,남녀비례위1.57:1.137주사문균분속우20충혈청형,기중장염사문균화서상한사문균위우세균형,분별점46.7%(64/137)화17.5%(24/137).공분71충PFGE대형,기중장염사문균화서상한사문균도유16충PFGE형별.장염사문균적4충PFGE형별(JEGX01.CN0001、JEGX01.CN0003、JFGX01.CN0002、JEGX01.CN0019)화서상한사문균적JPXX01.CN0001위우세분자형별. 결론 2008-2009년북경시사문균적류행구유성별、년령화계절성분포특정;PFGE분자형별교다,차존재차이명현적다개극륭계.
Objective To study the epidemiological characteristics and molecular phenotypes of Salmonella by pulsed-field gel electrophoresis (PFGE) in Beijing from 2008 to 2009. Methods A total of one hundred thirty-seven isolates recovered from the WHO Global Samonella Surveillance system and entero clinic surveillance system were identified by biochemical tests and serotyping.The related epidemiological informations were also analyzed.The isolates were further typed by PFGE. Results The prevalence of Salmonella from 2008 to 2009 showed obvious seasonal character.High incidence occurred from June to September,and 64.1% (84/131) isolates were recovered in this period.Patients of 18-40 year-old were 46.1% (58/128) and 80 patients were male and 40 patients were female with the ratio of 1.57:1.These 137 Salmonella isolates belonged to 20 serotypes,including Enteritidis (46.7%,64/137) and Typhimurium (17.5%,24/137) as the dominant serotype.In total,71 PFGE profiles were identified.Four PFGE patterns of S.Enteritidis isolates (JEGX01.CN0001,JEGX01.CN0003,JEGXO1.CN0002,JEGX01.CN0019) and S.Typhimurium pattern of JPXX01.CN0001 were dominant patterns. Conclusion The prevalence of Salmonella from 2008 to 2009 showed distribution characteristics of sex,age and seasons.The numerous PFGE patterns of Salmonella showed diversity of these isolates and different clones existed in Beijing.
