CAJ | 학술논문

胶原磺化羧甲基壳聚糖/硅橡胶双层人工皮肤%胶原壳聚糖/硅橡胶双层人工皮肤%脱细胞基质真皮支架%烧伤%新生血管化,生理性膠原磺化羧甲基殼聚糖/硅橡膠雙層人工皮膚%膠原殼聚糖/硅橡膠雙層人工皮膚%脫細胞基質真皮支架%燒傷%新生血管化,生理性
효원광화최갑기각취당/규상효쌍층인공피부%효원각취당/규상효쌍층인공피부%탈세포기질진피지가%소상%신생혈관화,생이성
Bilayer dermal equivalent composed of collagen- sulfonated carboxymethyl chitosan porous scaffold and silicone membrane%Bilayer dermal equivalent composed of collagen- chitosan porous scaffold and silicone membrane%Acellular dermal matrix%Burn%Neovasc

目的比较胶原壳聚糖真皮支架、胶原磺化羧甲基壳聚糖真皮支架及脱细胞基质真皮支架移植于Ⅲ度烧伤创面后,真皮支架的血管化及支架上表皮移植修复创面情况.方法将3种不同真皮支架各移植于6头猪(共18头猪)Ⅲ度烧伤清创后创面,在植入后1、2、3周对真皮支架血管化、创面、支架上表皮移植愈合和修复情况进行观察,同时用免疫组织化学方法,对CD34阳性信号(新生血管数目)进行检测.以无支架植入的Ⅲ度烧伤清创后创面为对照.结果胶原磺化羧甲基壳聚糖真皮支架植入后2周支架血管化已基本完成,而胶原壳聚糖和脱细胞基质真皮支架至少需要3周.3种不同材料支架垂直于创面的新生微血管均比无支架对照创面多;不同材料支架组与对照组2周创面比1周创面、3周创面比2周创面CD34的表达均明显增多,胶原磺化羧甲基壳聚糖真皮支架组植入后1、2、3周CD34阳性信号均明显高于相对应的其他3组;胶原磺化羧甲基壳聚糖真皮支架植入后1周,创面植表皮,移植表皮存活良好,而胶原壳聚糖和脱细胞基质真皮支架植入需要2周,其表面移植的表皮细胞才能成活.结论 3种材料的支架均可修复Ⅲ度烧伤创面,而以胶原磺化羧甲基壳聚糖真皮支架的血管化程度最好.
목적 비교효원각취당진피지가、효원광화최갑기각취당진피지가급탈세포기질진피지가이식우Ⅲ도소상창면후,진피지가적혈관화급지가상표피이식수복창면정황.방법 장3충불동진피지가각이식우6두저(공18두저)Ⅲ도소상청창후창면,재식입후1、2、3주대진피지가혈관화、창면、지가상표피이식유합화수복정황진행관찰,동시용면역조직화학방법,대CD34양성신호(신생혈관수목)진행검측.이무지가식입적Ⅲ도소상청창후창면위대조.결과 효원광화최갑기각취당진피지가식입후2주지가혈관화이기본완성,이효원각취당화탈세포기질진피지가지소수요3주.3충불동재료지가수직우창면적신생미혈관균비무지가대조창면다;불동재료지가조여대조조2주창면비1주창면、3주창면비2주창면CD34적표체균명현증다,효원광화최갑기각취당진피지가조식입후1、2、3주CD34양성신호균명현고우상대응적기타3조;효원광화최갑기각취당진피지가식입후1주,창면식표피,이식표피존활량호,이효원각취당화탈세포기질진피지가식입수요2주,기표면이식적표피세포재능성활.결론 3충재료적지가균가수복Ⅲ도소상창면,이이효원광화최갑기각취당진피지가적혈관화정도최호.
Objective To compare differences of angiogenesis among collagen- chitosan, collagensulfonated carboxymethyl chitosan porous scaffolds and acellular dermal matrix after these three different scaffolds with silicone membrane were transplanted on the wounds of full thickness burn, and the wound repair of different scaffolds with epidermis grafting on. Methods Angiogenesis in different dermal scaffolds, the wound surface and epidermis survival were observed in 1, 2, and 3 weeks after the three different scaffolds were respectively transplanted on wounds of full thickness burn with debriment in 6 Bama miniature pigs (total 18 pigs in 3 groups). At the same time, CD34 positive signals (neo-forming microvessels)were detected by immunohistochemical staining. The wounds without any scaffold transplantation were studied as the control. Results Angiogenesis had been fundamentally finished in 2 weeks after implantation of collagen- sulfonated carboxymethyl chitosan porous scaffold. And fundamental angiogenesis in collagen- chitosan porous scaffolds and acellular dermal matrix needed at least 3 weeks. Neo-forming micro-vessels perpendicular to wound beds with these three different scaffolds were more than those in the control wounds without scaffold. CD34 positive signals (neo-forming micro-vessels)were significantly higher in wounds at the second week than those in wounds at the first week. And those in wounds at the third week were significantly higher than those in wounds at the second week in all wounds with differentscaffold transplantations and the control wounds. CD34 positive signals in the group of sulfonated carboxymethyl chitosan porous scaffold on the 1st, 2nd and 3rd week after the scaffold transplantation were significantly higher than those corresponding signals in the other three groups. Epidermis on the sulfonated carboxymethyl chitosan porous scaffold which had been transplanted on burn wound for 1 week could survive perfectly, however, epidermis on the collagen- chitosan porous scaffold or acellular dermal matrix could not survive until these two scaffolds had been transplanted on the burn wounds for at least 2 weeks.Conclusions These three different scaffolds could repair the full thickness skin defects caused by burn, and angiogenesis of sulfonated carboxymethyl chitosan porous scaffold is the best.