CAJ | 학술논문

目的　探讨目前常见肌酐测定方法中存在的干扰。方法　以漂白土（fuller′s earth）吸附法为标准，对Jaffe′氏反应速率法、紫外法、比色法和电极法进行方法学评价。结果　在所采用的8种干扰物(丙酮酸钠、α-酮戊二酸、胆红素、血红蛋白、肌酸、多巴胺、抗坏血酸、地塞米松)中，Jaffe′氏反应速率法受干扰物影响最大，α-酮戊二酸(≥0.061 5 g/L)、抗坏血酸(≥0.82 mmol/L)、丙酮酸钠(≥0.186 mmol/L)对该法有正干扰，而胆红素(≥165.5 μmol/L)则有明显负干扰；紫外法基本不受干扰物影响；比色法不仅受肌酸(≥0.21 mmol/L)的正干扰，而且也受多巴胺(≥0.28 mmol/L)的负干扰；电极法仅受到肌酸(≥0.62 mmol/L)的正干扰。结论　紫外法是目前测定肌酐最好的方法。
목적　탐토목전상견기항측정방법중존재적간우。방법　이표백토（fuller′s earth）흡부법위표준，대Jaffe′씨반응속솔법、자외법、비색법화전겁법진행방법학평개。결과　재소채용적8충간우물(병동산납、α-동무이산、담홍소、혈홍단백、기산、다파알、항배혈산、지새미송)중，Jaffe′씨반응속솔법수간우물영향최대，α-동무이산(≥0.061 5 g/L)、항배혈산(≥0.82 mmol/L)、병동산납(≥0.186 mmol/L)대해법유정간우，이담홍소(≥165.5 μmol/L)칙유명현부간우；자외법기본불수간우물영향；비색법불부수기산(≥0.21 mmol/L)적정간우，이차야수다파알(≥0.28 mmol/L)적부간우；전겁법부수도기산(≥0.62 mmol/L)적정간우。결론　자외법시목전측정기항최호적방법。
Objective　To investigate the interference of common methods in determination of creatinine. Methods　Standardized with Fuller′s earth, Jaffe′s method, enzymatic method (ultraviolet), enzymatic method (colour), enzymatic method (electrode) were compared and evaluated. Results　In eight interference materials, Jaffe′s method was positively affected by pyruvic acid (≥0.186 mmol/L), α-ketoglutaric acid(≥0.0615 g/L), ascorbic acid(≥0.82 mmol/L) and negatively affected by bilirubin(≥165.5 μmol/L). Enzymatic method (ultraviolet) was not interfered. Enzymatic method (color) was positively affected by creatine(≥0.21 mmol/L) and negatively interfered by dopamine(≥0.28 mmol/L). At last, enzymatic method (electrode) was positively interfered by creatine(≥0.62 mmol/L). Conclusion　Enzymatic method(ultraviolet) is the best in the above methods.