CAJ | 학술논문

目的比较不同强度非消融聚焦超声预辐射对高强度聚焦超声(HIFU)疗效的影响,探讨提高HIFU消融效率的新方法.方法 40只肝VX2瘤兔分为预辐射组30只和对照组10只,预辐射组又分为60 W组、80 W组、100 W组,每组10只,采用不问功率较低强度HIFU预辐射处理;每组各取5只于次日行HIFU辐射,每组共辐射14条线段,24 h后取材行三苯氯化四氮唑(TTC)染色观察线性消融体积;其他动物于上述HIFU辐射的同时取材行苏木精伊红(HE)、琥珀酸脱氧酶(SDH)、TTC染色观察肿瘤组织结构和活性.结果 HIFU辐射后各组消融体积随预辐射强度增加而增大,能效因子(EEF)的变化则相反,其中100 W组消融效率最高;各组预辐射超声干预均未致肿瘤组织发生凝固性坏死,但80 W组和100 W组国组织结构和功能状态均有所改变,60 W组未见明显改变.结论适当强度的非消融超声预辐射能显著提高HIFU消融效率.
목적 비교불동강도비소융취초초성예복사대고강도취초초성(HIFU)료효적영향,탐토제고HIFU소융효솔적신방법.방법 40지간VX2류토분위예복사조30지화대조조10지,예복사조우분위60 W조、80 W조、100 W조,매조10지,채용불문공솔교저강도HIFU예복사처리;매조각취5지우차일행HIFU복사,매조공복사14조선단,24 h후취재행삼분록화사담서(TTC)염색관찰선성소융체적;기타동물우상술HIFU복사적동시취재행소목정이홍(HE)、호박산탈양매(SDH)、TTC염색관찰종류조직결구화활성.결과 HIFU복사후각조소융체적수예복사강도증가이증대,능효인자(EEF)적변화칙상반,기중100 W조소융효솔최고;각조예복사초성간예균미치종류조직발생응고성배사,단80 W조화100 W조국조직결구화공능상태균유소개변,60 W조미견명현개변.결론 괄당강도적비소융초성예복사능현저제고HIFU소융효솔.
Objective To assess the influence of pre-exposure to non-ablating ultrasound on the effect of high intensity focused ultrasound(HIFU)ablation.Methods Forty rabbits with transplanted VX2 tumors on their livers were divided into three pre-exposure groups(60 W,80 W and 100 W)and a control group(O W),with 10 rabbits in each group.Each group was pre-exposed to lower intensity focused ultrasound at the corresponding power.From each group,5 rabbits were randomly selected to be exposed to HIFU on the next day.Each group received 14scans.The rabbits were sacrificed 24 h later to take tissue samples for tfipheny tetrazolium chloride(TTC)staining to measure the amount of coagulative necrosis.Hematoxylin and eosin(HE)staining and succinate dehydrogenase (SDH)were also measured to observe the structure and activation of pre-exposed tumors.Results After HIFU exposure,the ablated volumes increased with pre-exposure acoustic intensity,and all volumes were larger than those in the control group.The ablating efficiency in the 100 W group was the highest.The pre-exposure did not itself ablate tumor tissue,but in the 80 W and 100 W groups the structure and activation of the tissues changed.Those in the 60 W group were not obviously altered.Conclusion Prior exposure to non-ablating ultrasound can highly enhance the efficacy of HIFU ablation.