中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
12期
1663-1664
,共2页
张明仪%肖春琦%沈文律%周昌艳%何新卫%郑重
張明儀%肖春琦%瀋文律%週昌豔%何新衛%鄭重
장명의%초춘기%침문률%주창염%하신위%정중
L-精氨酸%急性胰腺炎%模型,动物
L-精氨痠%急性胰腺炎%模型,動物
L-정안산%급성이선염%모형,동물
L-arginine%Acute pancreatitis%Model,animal
目的 建立L-精氨酸诱导大鼠暴发性胰腺炎动物模型.方法 用20%L-精氨酸(450 mg,120 ms/100 g大鼠体重)序贯腹腔和皮下给药建立大鼠暴发性胰腺炎模型.结果 实验大鼠胰腺有广泛变性、坏死,48 h达高峰,胰腺病理评分13.21±3.02;大鼠6～48 h死亡率为56.67%;血清淀粉酶和脂肪酶显著升高,皆12 h达到高峰,分别是(4120±759)、(1490±354)U/L;肺功、肝脏酶学和肾功能皆有显著性改变,48 h PaO2(78.24±3.56)InnlHg(1mmHg=0.133 kPa),48 h谷丙转氨酶(224.67±4.73)U/L,12 h肌酐(94.53±13.49)tunol/L;肺、肝、肾分别有轻到重度病理改变.结论 L-精氨酸能诱导出大鼠暴发性胰腺炎动物模型
目的 建立L-精氨痠誘導大鼠暴髮性胰腺炎動物模型.方法 用20%L-精氨痠(450 mg,120 ms/100 g大鼠體重)序貫腹腔和皮下給藥建立大鼠暴髮性胰腺炎模型.結果 實驗大鼠胰腺有廣汎變性、壞死,48 h達高峰,胰腺病理評分13.21±3.02;大鼠6～48 h死亡率為56.67%;血清澱粉酶和脂肪酶顯著升高,皆12 h達到高峰,分彆是(4120±759)、(1490±354)U/L;肺功、肝髒酶學和腎功能皆有顯著性改變,48 h PaO2(78.24±3.56)InnlHg(1mmHg=0.133 kPa),48 h穀丙轉氨酶(224.67±4.73)U/L,12 h肌酐(94.53±13.49)tunol/L;肺、肝、腎分彆有輕到重度病理改變.結論 L-精氨痠能誘導齣大鼠暴髮性胰腺炎動物模型
목적 건립L-정안산유도대서폭발성이선염동물모형.방법 용20%L-정안산(450 mg,120 ms/100 g대서체중)서관복강화피하급약건립대서폭발성이선염모형.결과 실험대서이선유엄범변성、배사,48 h체고봉,이선병리평분13.21±3.02;대서6～48 h사망솔위56.67%;혈청정분매화지방매현저승고,개12 h체도고봉,분별시(4120±759)、(1490±354)U/L;폐공、간장매학화신공능개유현저성개변,48 h PaO2(78.24±3.56)InnlHg(1mmHg=0.133 kPa),48 h곡병전안매(224.67±4.73)U/L,12 h기항(94.53±13.49)tunol/L;폐、간、신분별유경도중도병리개변.결론 L-정안산능유도출대서폭발성이선염동물모형
Objective To establish the rat model of L-arginine-induced fulminate acute pancreatitis (FAP). Methods FAP in Wistar male rats was induced by intraperitoneal injection of 20% L-Arg of 450 rag/100 g body weight and subcutaneous injections of 20% L-Arg of 120 mg/100 g body weight in an interval of 2 h. Results The extensive pancreatic necrosis and degeneration appeared in pancreatic exotrine tissue. Histological scores of pancreas were maximal to 13.21±3.02 at 48 h in rats with FAP. The mortality of rats with FAP was 56.67% during 6-48 h. The levels of serum amylase and lipase were significantly increased and reaehed the peak [ (4120±759) and (1490±354) U/L respectively] at 12 h. The levels of serum PaO2, ALT and Cr were significantly changed. PaO2 was (78.24±3.56 ) mmHg at 48 h, ALT (224.67±4.73 ) U/L at 48 h, and Cr (94.53±13.49) μmol/L at 12 h, respectively. Histological scores of lung and liver were Ⅱ - Ⅲ degrees, and those of kidney showed Ⅰ - Ⅱ degrees. Conclusion The model of FAP is induced by L-Arg in rats.
