CAJ | 학술논문

目的研究天然提取的内皮素拮抗剂对体外培养的B16鼠黑素瘤细胞的生物学作用.方法比较观察了内皮素拮抗剂和甘草黄酮对体外培养的B16鼠黑素瘤细胞酪氨酸酶活性、黑素含量和细胞增殖率的影响,以及内皮素拮抗剂对内皮素(ET-1)引起的B16细胞酪氨酸酶活性变化的作用.结果在实验浓度下,甘草黄酮具有浓度依赖性黑素合成抑制作用,内皮素拮抗剂对培养的B16黑素瘤细胞黑素生成没有直接的抑制作用,但能特异性抑制内皮素对黑素瘤细胞分化和酪氨酸酶的激活作用,200μg/mL该拮抗剂即可显著拮抗0.5μg/mL ET-1对黑素瘤细胞的刺激增殖作用,与甘草黄酮相比,细胞毒性较小.结论内皮素拮抗剂是一种安全的皮肤美白物质,在UVB照射后内皮素增加引起的皮肤色素沉着中,具有广泛的应用前景.
목적 연구천연제취적내피소길항제대체외배양적B16서흑소류세포적생물학작용.방법 비교관찰료내피소길항제화감초황동대체외배양적B16서흑소류세포락안산매활성、흑소함량화세포증식솔적영향,이급내피소길항제대내피소(ET-1)인기적B16세포락안산매활성변화적작용.결과 재실험농도하,감초황동구유농도의뢰성흑소합성억제작용,내피소길항제대배양적B16흑소류세포흑소생성몰유직접적억제작용,단능특이성억제내피소대흑소류세포분화화락안산매적격활작용,200μg/mL해길항제즉가현저길항0.5μg/mL ET-1대흑소류세포적자격증식작용,여감초황동상비,세포독성교소.결론 내피소길항제시일충안전적피부미백물질,재UVB조사후내피소증가인기적피부색소침착중,구유엄범적응용전경.
Objective To evaluate the biological effect of endothelin (ET) antagonist on cultured B16 murine melanoma cells. Methods B16 murine melanoma cells were cultured in the presence of various concentrations (31.25, 62.5, 125, 250, 500 μg/mL) of ET antagonist or licoflavone. Then, melanoma cells were harvested for the detection of tyrosinase activity and melanin content. The proliferation rate of melanoma cells was measured with MTT method. The effect of ET antagonist was compared with that of licoflavone. Results Licoflavone had a concentration-dependent inhibition on melanogenesis. The ET antagonist selectively suppressed the ET-induced stimulation of tyrosinase and cell differentiation of B16 cells, but had no direct inhibitory effect on melanogenesis in culture, and little influence on melanocyte viability. The addition of ET antagonist at 200 μg/mL could significantly inhibit ET (0.5 μg/mL)-induced melanogenesis in Bl6 cells. The cytotoxity of the antagonist was relatively lower than that of licoflavone. Conclusions The results suggest that the ET antagonist is a safe skin-whitening ingredient, and may have a wide application perspective in the prevention of endothelin-induced skin pigmentation after UVB irradiation.