中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2008年
12期
892-896
,共5页
陆佳琦%郑井红%张蕾%徐丛剑
陸佳琦%鄭井紅%張蕾%徐叢劍
륙가기%정정홍%장뢰%서총검
生殖器肿瘤,女(雌)性%肺肿瘤%甲氨蝶呤%输注,动脉内%大鼠
生殖器腫瘤,女(雌)性%肺腫瘤%甲氨蝶呤%輸註,動脈內%大鼠
생식기종류,녀(자)성%폐종류%갑안접령%수주,동맥내%대서
Genital neoplasms,female%Lung neoplasms%Methotrexate%Infusions,intraarterial%Rats
目的 研究甲氨蝶呤(MTX)髂内动脉灌注时大鼠肺及其他主要脏器药物浓度的变化.方法 选择50只雌性大鼠,随机分为2组,动脉组行股动脉插管、髂内动脉灌注药物,静脉组行股静脉插管、髂内静脉灌注药物.每组分别在5个时间点取血样及组织,利用建立的高效液相色谱方法 分析药物浓度.结果 动脉组大鼠肺、卵巢和子宫的药物浓度-时间曲线下面积(AUC)分别为(3.77±0.28)、(4.40±0.40)、(9.97±0.89)μg·h-1·g-1,静脉组分别为(2.31±0.25)、(3.91±0.19)、(7.65±1.54)μg·h-1·g-1,两组分别比较,差异均有统计学意义(P<0.05).动脉组大鼠血浆、心脏、肾、肝和脾的AUC分别为(6.13±0.53)、(1.90±0.11)、(5.32±0.89)、(14.16±1.96)、(0.76±0.20)μg·h-1·g-1,静脉组分别为(5.79±0.71)、(1.64±0.29)、(5.15±1.69)、(14.29±3.47)、(0.76±0.13)μg·h-1·g-1,两组分别比较,差异均无统计学意义(P>0.05).结论 相比较静脉给药,髂内动脉灌注给药后子宫、卵巢和肺有较高的药物浓度,髂内动脉灌注化疗町以有效地治疗妇科恶性肿瘤的肺转移.
目的 研究甲氨蝶呤(MTX)髂內動脈灌註時大鼠肺及其他主要髒器藥物濃度的變化.方法 選擇50隻雌性大鼠,隨機分為2組,動脈組行股動脈插管、髂內動脈灌註藥物,靜脈組行股靜脈插管、髂內靜脈灌註藥物.每組分彆在5箇時間點取血樣及組織,利用建立的高效液相色譜方法 分析藥物濃度.結果 動脈組大鼠肺、卵巢和子宮的藥物濃度-時間麯線下麵積(AUC)分彆為(3.77±0.28)、(4.40±0.40)、(9.97±0.89)μg·h-1·g-1,靜脈組分彆為(2.31±0.25)、(3.91±0.19)、(7.65±1.54)μg·h-1·g-1,兩組分彆比較,差異均有統計學意義(P<0.05).動脈組大鼠血漿、心髒、腎、肝和脾的AUC分彆為(6.13±0.53)、(1.90±0.11)、(5.32±0.89)、(14.16±1.96)、(0.76±0.20)μg·h-1·g-1,靜脈組分彆為(5.79±0.71)、(1.64±0.29)、(5.15±1.69)、(14.29±3.47)、(0.76±0.13)μg·h-1·g-1,兩組分彆比較,差異均無統計學意義(P>0.05).結論 相比較靜脈給藥,髂內動脈灌註給藥後子宮、卵巢和肺有較高的藥物濃度,髂內動脈灌註化療町以有效地治療婦科噁性腫瘤的肺轉移.
목적 연구갑안접령(MTX)가내동맥관주시대서폐급기타주요장기약물농도적변화.방법 선택50지자성대서,수궤분위2조,동맥조행고동맥삽관、가내동맥관주약물,정맥조행고정맥삽관、가내정맥관주약물.매조분별재5개시간점취혈양급조직,이용건립적고효액상색보방법 분석약물농도.결과 동맥조대서폐、란소화자궁적약물농도-시간곡선하면적(AUC)분별위(3.77±0.28)、(4.40±0.40)、(9.97±0.89)μg·h-1·g-1,정맥조분별위(2.31±0.25)、(3.91±0.19)、(7.65±1.54)μg·h-1·g-1,량조분별비교,차이균유통계학의의(P<0.05).동맥조대서혈장、심장、신、간화비적AUC분별위(6.13±0.53)、(1.90±0.11)、(5.32±0.89)、(14.16±1.96)、(0.76±0.20)μg·h-1·g-1,정맥조분별위(5.79±0.71)、(1.64±0.29)、(5.15±1.69)、(14.29±3.47)、(0.76±0.13)μg·h-1·g-1,량조분별비교,차이균무통계학의의(P>0.05).결론 상비교정맥급약,가내동맥관주급약후자궁、란소화폐유교고적약물농도,가내동맥관주화료정이유효지치료부과악성종류적폐전이.
Objective To establish the high-performance liquid chromatography(HPLC)method for measuring concentrations of methotrexate(MTX)in rat lung and some other tissues through internal iliac artery infusion.Methods Fifly female Sprague-Dawley rats were included in this study.The rats were randomly assigned to two groups.Methotrexate was injected to group one through internal iliac artery,and was injected to group two through femoral vein.Blood and tissues were collected in each group at 15,30,60.90 and 120 minutes for detection of the drug concentrations with HPLC.Results The area under the concentration time curve(AUC)in rat lung,ovary and uterus in the artery group were separately(3.77±0.28),(4.40±0.40),(9.97±0.89)μg·h-1·g-1,which were significantly different from those of the vein group[(2.31±0.25),(3.91±0.19),(7.65±1.54)μg·h-1·g-1;P<0.05].The AUC in the rat plasma,heart,kidney,liver and spleen in the artery group were separately(6.13±0.53),(1.90 ±0.11),(5.32±0.89),(14.16±1.96),(0.76±0.20)μg·h-1·g-1.There were no significant differences from the vein group[(5.79±0.71),(1.64±0.29),(5.15±1.69),(14.29 ±3.47),(0.76±0.13)μg·h-1·g-1;P>0.05].Conclusions Through internal iliac artery infusion,there are higher drug concentrations in lung.uterus and ovarian compared to venous injection.The internal-arterial chemotherapy may be used to treat pulmonary metastasis of gynecological tumor.
