国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
12期
1101-1103
,共3页
吴茱萸碱%吴茱萸次碱%复方吴茱萸软膏%高压液相色谱法
吳茱萸堿%吳茱萸次堿%複方吳茱萸軟膏%高壓液相色譜法
오수유감%오수유차감%복방오수유연고%고압액상색보법
Evodiamine%Rutaecarpine%Compound Wuzhuyuointment%HPLC
目的 建立同时测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱含量的方法.方法 采用HPLC法,色谱柱为Agilent HC-C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.08％辛烷磺酸钠(41∶59),流速为0.8 ml/min,柱温30℃,检测波长为225 nm.结果 吴茱萸碱和吴茱萸次碱分别在0.62～12.40 μg/ml (r=0.999 7)和0.24～4.80 μg/ml (r=0.9996)范围有良好的线性关系,平均加样回收率分别为97.9％、98.0％; RSD小于2.0％.结论 本方法快速简便,重复性好,可用于复方吴茱萸软膏的质量控制.
目的 建立同時測定複方吳茱萸軟膏中吳茱萸堿和吳茱萸次堿含量的方法.方法 採用HPLC法,色譜柱為Agilent HC-C18(250 mm×4.6 mm,5μm),流動相為乙腈-0.08％辛烷磺痠鈉(41∶59),流速為0.8 ml/min,柱溫30℃,檢測波長為225 nm.結果 吳茱萸堿和吳茱萸次堿分彆在0.62～12.40 μg/ml (r=0.999 7)和0.24～4.80 μg/ml (r=0.9996)範圍有良好的線性關繫,平均加樣迴收率分彆為97.9％、98.0％; RSD小于2.0％.結論 本方法快速簡便,重複性好,可用于複方吳茱萸軟膏的質量控製.
목적 건립동시측정복방오수유연고중오수유감화오수유차감함량적방법.방법 채용HPLC법,색보주위Agilent HC-C18(250 mm×4.6 mm,5μm),류동상위을정-0.08％신완광산납(41∶59),류속위0.8 ml/min,주온30℃,검측파장위225 nm.결과 오수유감화오수유차감분별재0.62～12.40 μg/ml (r=0.999 7)화0.24～4.80 μg/ml (r=0.9996)범위유량호적선성관계,평균가양회수솔분별위97.9％、98.0％; RSD소우2.0％.결론 본방법쾌속간편,중복성호,가용우복방오수유연고적질량공제.
Objective To establish a method for the determination of evodiamine and rutaecarpine of compound wuzhuyu ointment.Methods HPLC method was used with Agilent HC-C18(250 mm× 4.6 mm,5 μm) column and the mobile phase being acetonitrile-0.08％ ocane sutfonic acid sodium(41:59).The flow rate was 0.8 ml/min,the column temperature was 30℃,and the UV detector was set at 225 nm.Results The linear ranges of evodiamine and rutaecarpine were 0.62～ 12.40 μg/ml (r=0.9997) and 0.24～4.8 0 μg/ml (r=0.9996),respectively.The average recovery of evodiamine and rutaecarpine was 97.9％ and 98.0％,respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of evodiamine and rutaecarpine in compound Wuzhuyu ointment.
