中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
9期
1030-1033
,共4页
刘思兰%杨建平%王丽娜%刘磊%李彩芳%任春光%周静%李伟%江淼%马珍妮%邱桥成
劉思蘭%楊建平%王麗娜%劉磊%李綵芳%任春光%週靜%李偉%江淼%馬珍妮%邱橋成
류사란%양건평%왕려나%류뢰%리채방%임춘광%주정%리위%강묘%마진니%구교성
骨肿瘤%疼痛%NF-κB%白细胞介素6%肿瘤坏死因子α
骨腫瘤%疼痛%NF-κB%白細胞介素6%腫瘤壞死因子α
골종류%동통%NF-κB%백세포개소6%종류배사인자α
Bone neoplasms%Pain%NF-kappa B%Interleukin-6%Tumor necrosis factor-alpha
目的 评价骨癌痛大鼠脊髓NF-κB、IL-6和TNF-α表达的变化.方法 雌性健康SD大鼠72只,体重150～180 g,随机分为3组(n=24):对照组(C组)、假手术组(S组)和骨癌痛组(BP组).BP组于大鼠左侧胫骨干骺端骨髓腔内注射5μl Walker 256(1×105)乳腺癌细胞制备骨癌痛模型,S组左胫骨上端注射Hank液5μl.分别于肿瘤细胞接种前、接种后2、4、6、9、12、14、16、18和21 d时测定机械阈值.分别于肿瘤细胞接种后6、12和18 d时,处死3只大鼠,取L4-6脊髓组织,采用RT-PCR法测定NF-κB p65 mRNA、IL-6 mRNA和TNF-α mRNA的表达;另处死3只大鼠,取L4-6脊髓组织,计数NF-κBp65阳性细胞,采用免疫组化法测定NF-κB p65表达.结果 与C组和S组比较,BP组机械痛阈降低,脊髓NF-κB p65及其mRNA、IL-6 mRNA和TNF-αmRNA的表达上调,NF-κB p65阳性细胞计数增加(P＜0.05或0.01);C组和S组间上述指标比较差异无统计学意义(P＞0.05).结论 大鼠胫骨骨髓腔内注射肿瘤细胞通过激活脊髓NF-κB,导致炎性细胞因子IL-6和TNF-α大量释放,从而诱发骨癌痛.
目的 評價骨癌痛大鼠脊髓NF-κB、IL-6和TNF-α錶達的變化.方法 雌性健康SD大鼠72隻,體重150～180 g,隨機分為3組(n=24):對照組(C組)、假手術組(S組)和骨癌痛組(BP組).BP組于大鼠左側脛骨榦骺耑骨髓腔內註射5μl Walker 256(1×105)乳腺癌細胞製備骨癌痛模型,S組左脛骨上耑註射Hank液5μl.分彆于腫瘤細胞接種前、接種後2、4、6、9、12、14、16、18和21 d時測定機械閾值.分彆于腫瘤細胞接種後6、12和18 d時,處死3隻大鼠,取L4-6脊髓組織,採用RT-PCR法測定NF-κB p65 mRNA、IL-6 mRNA和TNF-α mRNA的錶達;另處死3隻大鼠,取L4-6脊髓組織,計數NF-κBp65暘性細胞,採用免疫組化法測定NF-κB p65錶達.結果 與C組和S組比較,BP組機械痛閾降低,脊髓NF-κB p65及其mRNA、IL-6 mRNA和TNF-αmRNA的錶達上調,NF-κB p65暘性細胞計數增加(P＜0.05或0.01);C組和S組間上述指標比較差異無統計學意義(P＞0.05).結論 大鼠脛骨骨髓腔內註射腫瘤細胞通過激活脊髓NF-κB,導緻炎性細胞因子IL-6和TNF-α大量釋放,從而誘髮骨癌痛.
목적 평개골암통대서척수NF-κB、IL-6화TNF-α표체적변화.방법 자성건강SD대서72지,체중150～180 g,수궤분위3조(n=24):대조조(C조)、가수술조(S조)화골암통조(BP조).BP조우대서좌측경골간후단골수강내주사5μl Walker 256(1×105)유선암세포제비골암통모형,S조좌경골상단주사Hank액5μl.분별우종류세포접충전、접충후2、4、6、9、12、14、16、18화21 d시측정궤계역치.분별우종류세포접충후6、12화18 d시,처사3지대서,취L4-6척수조직,채용RT-PCR법측정NF-κB p65 mRNA、IL-6 mRNA화TNF-α mRNA적표체;령처사3지대서,취L4-6척수조직,계수NF-κBp65양성세포,채용면역조화법측정NF-κB p65표체.결과 여C조화S조비교,BP조궤계통역강저,척수NF-κB p65급기mRNA、IL-6 mRNA화TNF-αmRNA적표체상조,NF-κB p65양성세포계수증가(P＜0.05혹0.01);C조화S조간상술지표비교차이무통계학의의(P＞0.05).결론 대서경골골수강내주사종류세포통과격활척수NF-κB,도치염성세포인자IL-6화TNF-α대량석방,종이유발골암통.
Objective To evaluate the changes in expression of NF-κB, IL-6 and TNF-α in spinal cord in a rat model of bone cancer. Methods Seventy-two female SD rats weighing 150-180 g were randomly divided into 3 groups (n = 24 each): control group (group C);sham operation group (group S) and bone cancer pain group (group BP). Bone cancer was induced by intra-tibial inoculation of 1 × 105 Walker 256 breast cancer cells. Paw withdrawal threshold to mechanical stimulation was measured with yon Frey filaments. The expression of NF-κB p65, IL-6 and TNF-α mRNA in the spinal cord was determined by RT-PCR and the expression of NF-κB p65 by immuno-histochemistry and NF-κB p65 positive cell count was determined. Results The paw withdrawal threshold was significantly lower and the expression of NF-κB p65, NF-κB p65 mRNA, IL-6 mRNA, TNF-α mRNA and NF-κB p65 positive cell count in the spinal cord were significantly higher in group BP than in group C and S ( P ＜0.05 or 0.01 ). Conclusion Intra-tibial inoculation of Walker 256 breast cancer cells activates NF-κB in the spinal cord, leading to the increased release of IL-6 and TNF-α and mechanical hyperalgesia.
