CAJ | 학술논문

从S418黑根霉，蓝色犁头霉，蓝色犁头霉D1、D2、D5，新月弯孢霉X1、X2和雅致小克银汉霉8种菌株中筛选出能高效转化生产C11β-羟基-16α，17α-环氧孕酮的菌株-雅致小克银汉霉，并经低能氮离子注入诱变，获得一株性能稳定的银汉霉突变株，在28℃下摇瓶发酵22h，48h后投料，C11β-羟基-16α，17α-环氧孕酮的转化率达到5．8％，比最初的转化率提高30％。
종S418흑근매，람색리두매，람색리두매D1、D2、D5，신월만포매X1、X2화아치소극은한매8충균주중사선출능고효전화생산C11β-간기-16α，17α-배양잉동적균주-아치소극은한매，병경저능담리자주입유변，획득일주성능은정적은한매돌변주，재28℃하요병발효22h，48h후투료，C11β-간기-16α，17α-배양잉동적전화솔체도5．8％，비최초적전화솔제고30％。
To breed microbial strain for C11β--hydroxylation of steroid, Cunningamella elegans was selected as an original strain from eight strains for induced mutagenesis, which including Rhizopus nigricans, Absidia coerulea, A bsidia coerulea （D1, D2 and D5）, Curvularidunata （X1 and X2） and Cunningamella elegans. The original induced strain was treated by low-energy nitrogen ion implantation mutagenesis and one strain of highly positive mutation was obtained. With 22 h fermentation at 28℃, the conversion rate of C11β-hydroxylation reaching 5.8%, enhanced by 30% compared with the initial of 4.6%.