CAJ | 학술논문

采用PCR和RACE技术从巴西橡胶树无性系热研7-33-97的乳管细胞中克隆到一条CXE羧酸酯酶蛋白家族基因HbCXE1,该基因cDNA全长1272 bp,含有一个长951 bp的阅读框,编码含316个氨基酸的HbCXE1蛋白。生物信息学分析表明,HbCXE1蛋白理论分子量为35.5437 ku,理论等电点为5.94,HbCXE1蛋白与蓖麻、杨树、葡萄、苹果、拟南芥的CXE蛋白同源性分别为75.08%、62.81%、62.31%、59.43%、57.68%。HbCXE1蛋白具有羧酸酯酶蛋白家族的特征结构,有一个保守的催化基团,由分布于一级结构基序中的不同位置的3个保守氨基酸[丝氨酸（位于保守的G-X-S-X-G结构域中间）、天冬氨酸/谷氨酸、组氨酸]组成,一个保守的氧离子洞HGG结构域,有8个β-折叠结构,5个α-螺旋结构,因此HbCXE1蛋白属于羧酸酯酶蛋白家族,可能参与橡胶树乳管细胞的防卫反应。
채용PCR화RACE기술종파서상효수무성계열연7-33-97적유관세포중극륭도일조CXE최산지매단백가족기인HbCXE1,해기인cDNA전장1272 bp,함유일개장951 bp적열독광,편마함316개안기산적HbCXE1단백。생물신식학분석표명,HbCXE1단백이론분자량위35.5437 ku,이론등전점위5.94,HbCXE1단백여비마、양수、포도、평과、의남개적CXE단백동원성분별위75.08%、62.81%、62.31%、59.43%、57.68%。HbCXE1단백구유최산지매단백가족적특정결구,유일개보수적최화기단,유분포우일급결구기서중적불동위치적3개보수안기산[사안산（위우보수적G-X-S-X-G결구역중간）、천동안산/곡안산、조안산]조성,일개보수적양리자동HGG결구역,유8개β-절첩결구,5개α-라선결구,인차HbCXE1단백속우최산지매단백가족,가능삼여상효수유관세포적방위반응。
In the present study,HbCXE1encoding a member of CXE protein family was cloned from laticifer of rubber tree clone Reyan7-33-97 by using PCR and RACE techniques.HbCXE1 was 1272 bp in length and contained an open reading frame of 951 bp encoding a putative protein of 316 amino acids.Bioinformatic analysis showed that： HbCXE1＇s theoretical molecular weight was 35.5437 ku,theoretical isoelectric point was 5.94;HbCXE1 shared amino acid sequence identity of 75.08%,62.81%,62.31%,59.43%,57.68% with the members of CXE protein family from Ricinus communis,Populus trichocarpa,Vitis vinifera,Malus pumila,and Arabidopsis thaliana,respectively;HbCXE1 contained the conserved structure of CXE protein family： the conserved catalytic triad,the oxyanion hole,a conserved structural core consisting of eight β-strands,with connecting α-helices and loops interspersed within and around the eight-β-strand core.So we deduced that HbCXE1 protein was a CXE protein and maybe play role in laticifers defense process.