CAJ | 학술논문

本试验比较了有机溶剂浸提法和超声波法提取总黄酮的效果，选择提取率较高的超声波法来提取。对料液比、超声时间和乙醇体积浓度对银杏中总黄酮提取效果的影响进行了单因子试验，并采用正交试验法找出最佳提取条件为料液比lg：30mL、超声时间30min、乙醇浓度70％。采用紫外可见分光法检测黄酮类化合物的含量，不加显色剂，直接以258nm作为最大吸收波长。结果表明，银杏中总黄酮的平均含量稳定在2．77％，精密度的RSD为0．302％，该方法的平均回收率为100．3％，本试验方法样品处理简单，准确度高，精密度好，适合于银杏叶中总黄酮的提取和测定。
본시험비교료유궤용제침제법화초성파법제취총황동적효과，선택제취솔교고적초성파법래제취。대료액비、초성시간화을순체적농도대은행중총황동제취효과적영향진행료단인자시험，병채용정교시험법조출최가제취조건위료액비lg：30mL、초성시간30min、을순농도70％。채용자외가견분광법검측황동류화합물적함량，불가현색제，직접이258nm작위최대흡수파장。결과표명，은행중총황동적평균함량은정재2．77％，정밀도적RSD위0．302％，해방법적평균회수솔위100．3％，본시험방법양품처리간단，준학도고，정밀도호，괄합우은행협중총황동적제취화측정。
This study discussed organic solvent extraction and ultrasonic extraction method. The extraction ratio of ultrasonic method was higher than the organic solvent extraction method when it was used for extraction of flavonoids. Single factor experiments were done with different extraction conditions including the ratio of Ginkgo biloba and ethanol, ultrasonic thne and the volume fractions of ethanol. The optimum extraction conditions of flavonoids from Ginkgo biloba were investigated by using orthogonal design.The results showed that lg：30 mL,70% ethanol and extraction for 30 min was the optimal condition. Quantitative analysis of flavonoids was achieved by ultraviolet spectrophotometry and do not need add colour - display reagent, detemfined at 258 nm directly as maximum absorption wavelength. The results indicated that the average content of flavonoids in Ginkgo biloba was 2.77 % under the optimal conditions, the relative standard deviation in precision test was t3. 302% ,and the average recovery rate was 100.3%. This study suggested that this method was simple, accurate, and also suitable for the extraction and quantitative analysis of tlavonoids in Ginkgo biloba.