CAJ | 학술논문

以分离自西藏那曲县罗玛镇传统发酵酸牦牛奶中的乳酸乳球菌乳脂亚种（Lactococcus lactis subsp．Cremoris）IMAU60064为试验菌株，研究其最佳的培养条件。对碳源、氮源、缓冲盐等培养基成分及培养条件进行优化，并采用响应面法对优选的碳源、氮源和缓冲盐类的组成含量进行优化，得到IMAU60064的增殖培养基为：葡萄糖23g／L、大豆蛋白胨11g／L、牛肉膏11g／L、胰蛋白胨5g／L、NaAC1．8g／L、K2HP041．2g／L、柠檬酸钠1．2g／L、MgSO4·7H200．4g／L、MnSO4·5H2O54mg／L、L-半胱氨酸盐酸盐0．5g/L、吐温80为1g／L。Lactococcus lactis subsp．cremorisIMAU60064在此增殖培养基中经30℃，14h培养活菌数可达到3．26×10^8cFu／mL，比在MRS中（6．54×10^7CFU／mL）提高近5倍。
이분리자서장나곡현라마진전통발효산모우내중적유산유구균유지아충（Lactococcus lactis subsp．Cremoris）IMAU60064위시험균주，연구기최가적배양조건。대탄원、담원、완충염등배양기성분급배양조건진행우화，병채용향응면법대우선적탄원、담원화완충염류적조성함량진행우화，득도IMAU60064적증식배양기위：포도당23g／L、대두단백동11g／L、우육고11g／L、이단백동5g／L、NaAC1．8g／L、K2HP041．2g／L、저몽산납1．2g／L、MgSO4·7H200．4g／L、MnSO4·5H2O54mg／L、L-반광안산염산염0．5g/L、토온80위1g／L。Lactococcus lactis subsp．cremorisIMAU60064재차증식배양기중경30℃，14h배양활균수가체도3．26×10^8cFu／mL，비재MRS중（6．54×10^7CFU／mL）제고근5배。
As one strain was isolated from yak milk in LuoMa Town of Nagqu Prefecture of Tibet, Lactococcus lactis subsp. cremoris IMAU60064 was studied for its optimal condition of growth. Medium components such as carbon sources, nitrogen sources and cultivation condition were optimized, the optimal concentration of carbon sources, nitrogen sources and buffer salts were determined by using response surface methodology. The results showed that Lactococcus lactis subsp, cremoris IMAU60064 optimal culture medium components was consist of 23 g/L glucose, 11 g/L soy peptone, 11 g/L beef extract, 5 g/L tryptone, 1.8 g/L sodium acetate, 1.2 g/L K2HPO4, 1.2 g/L sodium citrate, 0.4 g/L MgSO4·7H2O, 54 mg/L MnSO4·5H20, 0.5 g/L L-cysteine, 1 g/L Tween-80. After cultivated in enrichment medium for 14 h at 30℃, the living cells ofLactococcus lactis subsp. cremoris IMAU60064 were 3.26×10^8 CFU/mL, which was about 5 times higher than in MRS （6.54× 10^7 CFU/mL）. Keywords： Lactococcus lactis subsp, cremoris IMAU60064, enrichment medium, optimization