CAJ | 학술논문

具有多选择标记的植物基因表达载体有利于转基因植物研究中的转基因植株的筛选。本研究对植物基因表达载体pCAMBIA1301进行了改造,产生了1个具有可溶性的红移绿色荧光蛋白基因（smRS-GFP）、抗除草剂Basta、葡萄糖苷酸酶（GUS）及潮霉素（Hpt）的多选择标记的新的植物基因表达载体。运用这一表达载体的多选择标记可以有效降低检测和筛选转化植株时的假阳性率。此外,如此的基因表达载体也能满足实验室的不同筛选方法的需求。
구유다선택표기적식물기인표체재체유리우전기인식물연구중적전기인식주적사선。본연구대식물기인표체재체pCAMBIA1301진행료개조,산생료1개구유가용성적홍이록색형광단백기인（smRS-GFP）、항제초제Basta、포도당감산매（GUS）급조매소（Hpt）적다선택표기적신적식물기인표체재체。운용저일표체재체적다선택표기가이유효강저검측화사선전화식주시적가양성솔。차외,여차적기인표체재체야능만족실험실적불동사선방법적수구。
A plant gene expression vector with multi-selectable markers benefits screening of transgenic plants in plant transgene researches. In this study, a plant gene expression vector pCAMBIA1301 was re-constructed, generating a new plant expression vector with multi-selectable markers of red shift green fluorescent protein （smRS-GFP） gene, herbicide Basta, and hygromycin （Hpt）. Use of multi-selectable markers of the expression vector would efficiently reduce false positive rate in detecting and screening of transformed plants. Additionally, such gene expression vector can also meet the needs of different screening methods in laboratories.