基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
Genomics and Applied Biology
2011年
2期
204-211
,共8页
孙润泽%侯琦%章文乐%钮俊%张志毅%林善枝
孫潤澤%侯琦%章文樂%鈕俊%張誌毅%林善枝
손윤택%후기%장문악%뉴준%장지의%림선지
甜杨%microRNA%低温胁迫%表达谱
甜楊%microRNA%低溫脅迫%錶達譜
첨양%microRNA%저온협박%표체보
Populus suaveolens%miRNA%Low temperature stress%Expression profile
MicroRNAs(miRNAs)作为一类21碱基左右的非编码小RNAs,参与植物生长发育的调控,并在植物对生物与非生物胁迫的应答过程中发挥重要作用。本研究依据miRNA高度保守特点,利用已公布的毛果杨(Populus trichocarpa)基因组序列设计引物,从甜杨(Populus suaveolens)基因组中克隆获得了12个miRNA基因座序列。序列比对结果表明,这些miRNA基因均为毛果杨低温响应miRNA基因的同源序列。同时,以低温(0℃)处理0~48h的甜杨幼苗为试材,通过半定量RT-PCR法对miRNA基因的成熟体序列在不同处理时间下的表达谱进行分析,结果显示,大多数miRNA成熟体序列在甜杨低温胁迫下的表达模式与其在毛果杨中的表达极为相似,由此可推测这些保守性miRNAs可能在甜杨和毛果杨两物种对低温胁迫的应答反应中发挥相似的功能,而miR168a、miR168b和miR475a在两物种间表达现象的差异,表明它们可能通过调控多种靶基因而发挥不同作用。本文结果将为进一步研究甜杨基因功能提供基础。
MicroRNAs(miRNAs)作為一類21堿基左右的非編碼小RNAs,參與植物生長髮育的調控,併在植物對生物與非生物脅迫的應答過程中髮揮重要作用。本研究依據miRNA高度保守特點,利用已公佈的毛果楊(Populus trichocarpa)基因組序列設計引物,從甜楊(Populus suaveolens)基因組中剋隆穫得瞭12箇miRNA基因座序列。序列比對結果錶明,這些miRNA基因均為毛果楊低溫響應miRNA基因的同源序列。同時,以低溫(0℃)處理0~48h的甜楊幼苗為試材,通過半定量RT-PCR法對miRNA基因的成熟體序列在不同處理時間下的錶達譜進行分析,結果顯示,大多數miRNA成熟體序列在甜楊低溫脅迫下的錶達模式與其在毛果楊中的錶達極為相似,由此可推測這些保守性miRNAs可能在甜楊和毛果楊兩物種對低溫脅迫的應答反應中髮揮相似的功能,而miR168a、miR168b和miR475a在兩物種間錶達現象的差異,錶明它們可能通過調控多種靶基因而髮揮不同作用。本文結果將為進一步研究甜楊基因功能提供基礎。
MicroRNAs(miRNAs)작위일류21감기좌우적비편마소RNAs,삼여식물생장발육적조공,병재식물대생물여비생물협박적응답과정중발휘중요작용。본연구의거miRNA고도보수특점,이용이공포적모과양(Populus trichocarpa)기인조서렬설계인물,종첨양(Populus suaveolens)기인조중극륭획득료12개miRNA기인좌서렬。서렬비대결과표명,저사miRNA기인균위모과양저온향응miRNA기인적동원서렬。동시,이저온(0℃)처리0~48h적첨양유묘위시재,통과반정량RT-PCR법대miRNA기인적성숙체서렬재불동처리시간하적표체보진행분석,결과현시,대다수miRNA성숙체서렬재첨양저온협박하적표체모식여기재모과양중적표체겁위상사,유차가추측저사보수성miRNAs가능재첨양화모과양량물충대저온협박적응답반응중발휘상사적공능,이miR168a、miR168b화miR475a재량물충간표체현상적차이,표명타문가능통과조공다충파기인이발휘불동작용。본문결과장위진일보연구첨양기인공능제공기출。
MicroRNAs (miRNAs) are ~21-nucleotide non-protein-coding small RNAs that play key roles in plant development and response to abiotic stresses.In this paper,12 homologous sequences of cold stress-responsive Ptc-miRNA genes were isolated from the genomic DNA of Populus suaveolens based on conservation of miRNA within and between species.Additionally,the expression profiles of the obtained mature miRNAs from P.suave-olens cuttings exposed to low temperature (0℃) for 0 to 48 hours were analyzed by semi-quantitative RT-PCR.The results showed that the little difference in expression profiles of most mature miRNAs under cold stress between P.suaveolens and P.trichocarpa,suggesting that these conserved miRNAs may play similar roles in response to low temperature stress,while the different expression profiles of miR168a,miR168b and miR475a indicated that they might have different target genes between these two species.The results of this paper would provide some groundwork for further investigating gene function of Populus suaveolens.