广东畜牧兽医科技
廣東畜牧獸醫科技
엄동축목수의과기
GUANDONG JOURNAL OF ANIMAL AND VETERINARY SCIENCE
2011年
4期
28-31
,共4页
古飞霞%田云%胡婉怡%刘容珍
古飛霞%田雲%鬍婉怡%劉容珍
고비하%전운%호완이%류용진
珍珠鸡%大肠杆菌%鉴定%16S%rRNA
珍珠鷄%大腸桿菌%鑒定%16S%rRNA
진주계%대장간균%감정%16S%rRNA
Guinea-fowl%Escherila coli%Identification%16S rRNA
对来源于广东省珍禽养殖场的3株珍珠鸡源大肠杆菌进行了形态特征、培养特性、生化特性、药敏特性和致病性鉴定,同时对16S rRNA序列进行比较分析。结果发现3株分离茵均对珍珠鸡有较强致病性,但是3株细菌对乳糖、棉子糖、蔗糖、赖氨酸的利用存在差异,对庆大霉素、青霉素、红霉素、环丙沙星、四环素、利福平和氟哌酸等药物的敏感性存在较大差别。3个16S rRNA序列之间有11个位点的不同,经DNAstar软件分析后发现,它们均位于不同的分支中。
對來源于廣東省珍禽養殖場的3株珍珠鷄源大腸桿菌進行瞭形態特徵、培養特性、生化特性、藥敏特性和緻病性鑒定,同時對16S rRNA序列進行比較分析。結果髮現3株分離茵均對珍珠鷄有較彊緻病性,但是3株細菌對乳糖、棉子糖、蔗糖、賴氨痠的利用存在差異,對慶大黴素、青黴素、紅黴素、環丙沙星、四環素、利福平和氟哌痠等藥物的敏感性存在較大差彆。3箇16S rRNA序列之間有11箇位點的不同,經DNAstar軟件分析後髮現,它們均位于不同的分支中。
대래원우광동성진금양식장적3주진주계원대장간균진행료형태특정、배양특성、생화특성、약민특성화치병성감정,동시대16S rRNA서렬진행비교분석。결과발현3주분리인균대진주계유교강치병성,단시3주세균대유당、면자당、자당、뢰안산적이용존재차이,대경대매소、청매소、홍매소、배병사성、사배소、리복평화불고산등약물적민감성존재교대차별。3개16S rRNA서렬지간유11개위점적불동,경DNAstar연건분석후발현,타문균위우불동적분지중。
The morphological character, cultural character, biochemical characteristic, drug sensitivity and pathogenicity of three Escherila coli strains isolated fi'om guinea-fowl farms of Guangdong province were detected and the 16S rRNA of these strains were compared. The results showed that three strains were pathogenic to guinea fowls. The fermentation to lactose, raffinose, sucrose and lysine were different. The sensitivity to gentamicin, penicillin, erythrocin, ciprofloxacin, tetracycline, rifampicin and orfloxacin were different also. Eleven different nucleotide acid sites were found on three 16S rRNAs. Three 16S rRNAs were clustered into three different branches.