CAJ | 학술논문

目的观察硅胶管包裹所致的血管外膜损伤对大鼠颈动脉收缩功能的影响。方法用硅胶管经血管外膜包裹大鼠颈动脉，分别于术后3d、1周、2周测量大鼠双侧颈动脉血流量，观察血管对局部应用5一羟色胺（5－HT）的反应，光镜下观察血管形态学变化。结果与对照侧比较，硅胶管包裹大鼠颈动脉的早期阶段，包管侧颈动脉呈血管慢性收缩的组织学改变，表现为血管腔缩小[包管3d缩小（12．15±2．29）％（P=0．003）；包管1周缩小（45．17±3．84）％（P〈0．001）]。内弹力板弯曲、中膜增厚[包管3d增厚（23．04＋5．96）％（P=0．009）；包管1周增厚（61．65±10．32）％（P〈0．001）]，伴颈动脉血流量降低及血管对5-HT的收缩反应增强。硅胶管包裹2周，包管侧颈动脉管壁重塑，表现为中膜增厚[增厚（31．52±4．56）％（P=0．012）]及弥漫性血管内膜增生[新生内膜面积平均（0．19±0．05）mm^2]，伴血管腔面积缩小[减少（37．17±4．57）％（P〈0．001）]及颈动脉血流量降低，血管对5-HT的收缩反应恢复至对照侧水平。结论血管外膜损伤能引起血管收缩功能增强及新生内膜形成，血管收缩功能的改变出现在内膜增生性病变之前。
목적관찰규효관포과소치적혈관외막손상대대서경동맥수축공능적영향。방법용규효관경혈관외막포과대서경동맥，분별우술후3d、1주、2주측량대서쌍측경동맥혈류량，관찰혈관대국부응용5일간색알（5－HT）적반응，광경하관찰혈관형태학변화。결과여대조측비교，규효관포과대서경동맥적조기계단，포관측경동맥정혈관만성수축적조직학개변，표현위혈관강축소[포관3d축소（12．15±2．29）％（P=0．003）；포관1주축소（45．17±3．84）％（P〈0．001）]。내탄력판만곡、중막증후[포관3d증후（23．04＋5．96）％（P=0．009）；포관1주증후（61．65±10．32）％（P〈0．001）]，반경동맥혈류량강저급혈관대5-HT적수축반응증강。규효관포과2주，포관측경동맥관벽중소，표현위중막증후[증후（31．52±4．56）％（P=0．012）]급미만성혈관내막증생[신생내막면적평균（0．19±0．05）mm^2]，반혈관강면적축소[감소（37．17±4．57）％（P〈0．001）]급경동맥혈류량강저，혈관대5-HT적수축반응회복지대조측수평。결론혈관외막손상능인기혈관수축공능증강급신생내막형성，혈관수축공능적개변출현재내막증생성병변지전。
Objective To observe the effects of the chronic adventitia injury on the vasoconstriction of the rat carotid artery. Methods A non-occlusive silicone collar was positioned around the right carotid artery of rats. Blood flow and vascular reactivity to 5-HT were examined, and both carotids were harvested for morphometry at day 3,7 and 14 after operation. Results In the early stage of advenfitia injury induced by positioning a silicone collar around file rat carotid artery, there appeared the characteristic histological changes of chronic vasospasm in collared artery, such as the reduction of the luminal area for （12.15±2.29）% at day 3 after operation （P =0.003 ） and （45.17±3.84）% at day 7 （P 〈 0.001 ）] ,corrugation of the internal elastic lamina,medial thickening up to [ （23.04±5.96）% at day 3 （P =0.009）, （61.65±10.32）% for day 7 （P 〈 0.001 ）] ,the reduction of the blood flow and the increase of vascular reactivity to 5-HT when compared to con- tralateral arteries. Two weeks after collar placement, the vascular wall remodeling was observed in injured artery, such as the medial thickening for [（31.52±4.56） %,P =0.012] and a diffuse intimal hyperplasia,the reduction of the lunfinal area [（37.17±4.57）% （P 〈 0.001）] and the carotid artery blood flow. The average neointima area was （0.19±0.05） rom2 in collared arteries. The vascular reactivity to 5-HT came back to the normal level. Conclusions Collar-induced advenfitia injury caused the enhancement of vascular contractility and the neointima formation. The change of vascular contractility appeared before the formation of neointima.