CAJ | 학술논문

探讨中华补血草水提物(LSE)对抗N-乙酰对氨基酚(N-acetyl-p-aminophenol,APAP)引起的肝细胞损伤的作用及其可能的线粒体机制.通过分组实验,测定血清中谷丙、谷草转氨酶(SALT,sAST)的活性和谷胱甘肽(GSH)的含量,同时制备和观察肝组织切片.其后进一步探讨线粒体在这一过程中所发生的变化,通过测定线粒体的肿胀、线粒体膜电位(MMP)的变化以及电压依赖性离子通道(VDAC)在转录水平的变化等,探讨其相应的线粒体作用机制.结果显示:100、200、400 mg/kg的LSE可以剂量依赖性地对抗A:PAP引起的肝细胞损伤,而这种护肝活性可能与LSE对肝细胞线粒体的保护作用有关.同时还发现,线粒体的一个重要的外膜蛋白--VDAC的表达可能与之有着密切的关系.
탐토중화보혈초수제물(LSE)대항N-을선대안기분(N-acetyl-p-aminophenol,APAP)인기적간세포손상적작용급기가능적선립체궤제.통과분조실험,측정혈청중곡병、곡초전안매(SALT,sAST)적활성화곡광감태(GSH)적함량,동시제비화관찰간조직절편.기후진일보탐토선립체재저일과정중소발생적변화,통과측정선립체적종창、선립체막전위(MMP)적변화이급전압의뢰성리자통도(VDAC)재전록수평적변화등,탐토기상응적선립체작용궤제.결과현시:100、200、400 mg/kg적LSE가이제량의뢰성지대항A:PAP인기적간세포손상,이저충호간활성가능여LSE대간세포선립체적보호작용유관.동시환발현,선립체적일개중요적외막단백--VDAC적표체가능여지유착밀절적관계.
; The present study was undertaken to investigate the hepatoprotective activity of aqueous extracts of Limonium sinense (Girard) Ktze root (LSE) against acetaminophen (APAP)-induced liver damage and to elucidate the possible mechanisms underlying the hepatoprotection. The serum alanine aminotransferase (sALT), serum aspartate aminotransferase (sAST) activity and serum glutathione (GSH) content were detected, and the histopathology of liver was observed. The mitochondrial swelling, mitochondrial membrane potential (MMP) and transcription of voltage-dependent anion channels (VDAC) gene were also investigated. It was found that 100, 200and 400mg/kg LSE could restore the APAP-induced changes on mice liver in a dose-dependent manner. And the mechanisms underlying its hepatoprotection might be related to the protection on liver mitochondria, especially on VDAC, the most important protein on outer membrane of mitochondria.