肿瘤
腫瘤
종류
TUMOR
2010年
1期
68-72
,共5页
张慧%范志松%刘巍%孟宪利%李保庆%刘俊峰%吴国祥%陈勇%左静%吕雅蕾%王玉栋
張慧%範誌鬆%劉巍%孟憲利%李保慶%劉俊峰%吳國祥%陳勇%左靜%呂雅蕾%王玉棟
장혜%범지송%류외%맹헌리%리보경%류준봉%오국상%진용%좌정%려아뢰%왕옥동
食管肿瘤%反转录聚合酶链试验%血液学试验%基因,ERCC1,ERCC2
食管腫瘤%反轉錄聚閤酶鏈試驗%血液學試驗%基因,ERCC1,ERCC2
식관종류%반전록취합매련시험%혈액학시험%기인,ERCC1,ERCC2
Esophageal neoplasms%Reverse transcriptase polymerase chain reaction%Hematologic test%Gene,ERCC1,ERCC2
目的:探讨检测食管鳞状细胞癌患者外周血替代肿瘤组织中切除修复交叉互补基因1(excision repair cross-complementing 1,ERCC1)及ERCC2表达的可行性.方法:收集39例食管癌患者的术前外周血、肿瘤组织及癌旁正常组织,应用RT-PCR法检测ERCC1和ERCC2 mRNA的表达,应用ELISA法检测血清蛋白中ERCC1和ERCC2的含量,以10名健康志愿者外周血作为对照.结果: ERCC1和ERCC2 mRNA以及蛋白在健康志愿者外周血中的表达高于食管癌患者外周血(P<0.05);食管癌患者外周血中ERCC1和ERCC2 mRNA的表达水平与其在肿瘤组织中的表达水平呈明显的正相关关系(P<0.01).结论:食管鳞状细胞癌患者外周血中ERCC1和ERCC2 mRNA的表达水平能在一定程度上间接反映其在肿瘤组织中的表达水平.
目的:探討檢測食管鱗狀細胞癌患者外週血替代腫瘤組織中切除脩複交扠互補基因1(excision repair cross-complementing 1,ERCC1)及ERCC2錶達的可行性.方法:收集39例食管癌患者的術前外週血、腫瘤組織及癌徬正常組織,應用RT-PCR法檢測ERCC1和ERCC2 mRNA的錶達,應用ELISA法檢測血清蛋白中ERCC1和ERCC2的含量,以10名健康誌願者外週血作為對照.結果: ERCC1和ERCC2 mRNA以及蛋白在健康誌願者外週血中的錶達高于食管癌患者外週血(P<0.05);食管癌患者外週血中ERCC1和ERCC2 mRNA的錶達水平與其在腫瘤組織中的錶達水平呈明顯的正相關關繫(P<0.01).結論:食管鱗狀細胞癌患者外週血中ERCC1和ERCC2 mRNA的錶達水平能在一定程度上間接反映其在腫瘤組織中的錶達水平.
목적:탐토검측식관린상세포암환자외주혈체대종류조직중절제수복교차호보기인1(excision repair cross-complementing 1,ERCC1)급ERCC2표체적가행성.방법:수집39례식관암환자적술전외주혈、종류조직급암방정상조직,응용RT-PCR법검측ERCC1화ERCC2 mRNA적표체,응용ELISA법검측혈청단백중ERCC1화ERCC2적함량,이10명건강지원자외주혈작위대조.결과: ERCC1화ERCC2 mRNA이급단백재건강지원자외주혈중적표체고우식관암환자외주혈(P<0.05);식관암환자외주혈중ERCC1화ERCC2 mRNA적표체수평여기재종류조직중적표체수평정명현적정상관관계(P<0.01).결론:식관린상세포암환자외주혈중ERCC1화ERCC2 mRNA적표체수평능재일정정도상간접반영기재종류조직중적표체수평.
Objective:To investigate the feasibility of detecting excision repair cross-complementing 1(ERCC1)and ERCC2 in peripheral venous blood instead of cancer tissues from esophageal squamous cell carcinoma patients. Methods:The expressions of ERCC1 and ERCC2 mRNA were detected by using RT-PCR in 39 cases of peripheral venous blood samples, esophageal squamous cell carcinoma tissues, and adjacent normal tissues. ELISA was used to determine the levels of ERCC1 and ERCC2 proteins in serum. The periphe-ral blood from 10 healthy volunteers was used as control. Results:Expression levels of ERCC1 and ERCC2 mRNA and protein were significantly higher in peripheral blood from healthy control than those in esophageal carcinoma patients (P<0.05). There was a positive correlation between the expression of ERCC1 and ERCC2 mRNA in peripheral blood and cancer tissues (P<0.01). Conclusion:The expression levels of ERCC1 and ERCC2 mRNA in peripheral blood can indirectly reflect their expression levels in human esophageal squamous cell carcinoma tissues.
