热带作物学报
熱帶作物學報
열대작물학보
CHINESE JOURNAL OF TROPICAL CROPS
2009年
12期
1858-1864
,共7页
蔡学清%林通%谢玲平%吴昌镇%林娜%胡方平
蔡學清%林通%謝玲平%吳昌鎮%林娜%鬍方平
채학청%림통%사령평%오창진%림나%호방평
荔枝%酸腐菌%生物学特性
荔枝%痠腐菌%生物學特性
려지%산부균%생물학특성
lychee%Geotrichum candidum%biological characteristics.
对福建省分离的荔枝酸腐病菌的形态学特征、生长速度、产孢、分生孢子萌发及侵染的影响因子进行研究.结果表明:福建荔枝酸腐病的病原菌为白地霉(Geotrichum candidum);该菌最适生长温度为24~28℃,pH值为8~9,光照时间为12~20h,最适生长与产孢培养基为PDA,分生孢子致死温度为65℃,10min;该菌分生孢子的萌发率与pH值、培养基、光照时间、温度及营养成分等有关,其中该菌在24~32℃,光照时间为16~20h,培养15 d时所产生的分生孢子萌发率最高;该菌不同培养时间所产生的分生孢子及不同温度条件对其侵染荔枝的能力也不同,培养10d所产生的分生孢子侵染力最强.侵染最适温度为28~32℃.该菌除了寄生荔枝外,人工接种还能侵染胡萝卜、番茄、水蜜桃、龙跟、葡萄、茄子和黄瓜.试验结果为进一步了解该病的发生危害提供理论依据.
對福建省分離的荔枝痠腐病菌的形態學特徵、生長速度、產孢、分生孢子萌髮及侵染的影響因子進行研究.結果錶明:福建荔枝痠腐病的病原菌為白地黴(Geotrichum candidum);該菌最適生長溫度為24~28℃,pH值為8~9,光照時間為12~20h,最適生長與產孢培養基為PDA,分生孢子緻死溫度為65℃,10min;該菌分生孢子的萌髮率與pH值、培養基、光照時間、溫度及營養成分等有關,其中該菌在24~32℃,光照時間為16~20h,培養15 d時所產生的分生孢子萌髮率最高;該菌不同培養時間所產生的分生孢子及不同溫度條件對其侵染荔枝的能力也不同,培養10d所產生的分生孢子侵染力最彊.侵染最適溫度為28~32℃.該菌除瞭寄生荔枝外,人工接種還能侵染鬍蘿蔔、番茄、水蜜桃、龍跟、葡萄、茄子和黃瓜.試驗結果為進一步瞭解該病的髮生危害提供理論依據.
대복건성분리적려지산부병균적형태학특정、생장속도、산포、분생포자맹발급침염적영향인자진행연구.결과표명:복건려지산부병적병원균위백지매(Geotrichum candidum);해균최괄생장온도위24~28℃,pH치위8~9,광조시간위12~20h,최괄생장여산포배양기위PDA,분생포자치사온도위65℃,10min;해균분생포자적맹발솔여pH치、배양기、광조시간、온도급영양성분등유관,기중해균재24~32℃,광조시간위16~20h,배양15 d시소산생적분생포자맹발솔최고;해균불동배양시간소산생적분생포자급불동온도조건대기침염려지적능력야불동,배양10d소산생적분생포자침염력최강.침염최괄온도위28~32℃.해균제료기생려지외,인공접충환능침염호라복、번가、수밀도、룡근、포도、가자화황과.시험결과위진일보료해해병적발생위해제공이론의거.
An isolate collected from lychee fruit infected with sour rot in Fujian was detected in terms of mycelial growth, sporulation and conidial germination for pathogenic identification, and the factors influencing pathogenic infection were also observed. This isolate was identified as Geotrichum candidum. For the mycelial growth of G. candidum, the optimum growth conditions were that temperature ranged from 24 to 28℃, pH 8-9 and illumination 12~20 h. The best medium for the growth and sporulation of the fungus was PDA. The lethal temperature for conidia was 65℃. Moreover, the effects of pH, medium, illumination duration, temperature and nutrients on the conidial germination of the fungus were studied. The results showed that the eonidia cultured for 15d at 24~32℃ with 16-20 h of illumination had the highest germination rate. And, the eonidia having different ages or at different temperatures had different capacities infecting lychee fruit. When cultured for 10 d, the conidia had the strongest infectivity, and the optimum temperature for their infection ranged from 28 to 32℃. Apart from lychee, this pathogen could also infect carrot, tomato, peach, longan, grape, eggplant and cucumber under artificial inoculation. This experiment provides a theoretical basis for further understanding of the occurrence and damage of the litchi sour rot disease.