CAJ | 학술논문

目的探讨宫内缺氧对新生大鼠大脑皮质神经元与VEGF mRNA表达的影响以及当归的调控作用.方法孕14 d健康SD雌性大鼠15只,随机分为对照组、缺氧组和当归组各5只,于孕14 d开始将当归组与缺氧组孕鼠置于低张氧浓度三气培养箱中,制作胎鼠宫内缺氧模型,此前一小时按8 mL/kg分别给予当归和生理盐水尾静脉注射,对照组不缺氧,余同缺氧组.三组孕鼠分娩当日每窝随机选取新生鼠4只,取脑组织多聚甲醛固定,石蜡包埋切片、NSE mRNA、VEGF mRNA原位杂交,400倍拍照、IPP6.0软件图像分析.结果缺氧组新生大鼠大脑皮质NSE mRNA阳性细胞数较对照组减少,积分光密度值(IOD)降低(P＜0.05),VEGF mRNA阳性细胞IOD值升高(P＜0.05);当归组新生大鼠大脑皮质NSE mRNA阳性细胞数较缺氧组增多、IOD值增高(P＜0.05),VEGF mRNA阳性细胞IOD值增高(P＜0.05).结论宫内缺氧可致新生大鼠大脑皮质神经元受损,当归注射液对此损伤有一定保护作用,其机制可能是通过上调大脑皮质VEGF mRNA的表达而使缺氧环境改善.
목적 탐토궁내결양대신생대서대뇌피질신경원여VEGF mRNA표체적영향이급당귀적조공작용.방법 잉14 d건강SD자성대서15지,수궤분위대조조、결양조화당귀조각5지,우잉14 d개시장당귀조여결양조잉서치우저장양농도삼기배양상중,제작태서궁내결양모형,차전일소시안8 mL/kg분별급여당귀화생리염수미정맥주사,대조조불결양,여동결양조.삼조잉서분면당일매와수궤선취신생서4지,취뇌조직다취갑철고정,석사포매절편、NSE mRNA、VEGF mRNA원위잡교,400배박조、IPP6.0연건도상분석.결과 결양조신생대서대뇌피질NSE mRNA양성세포수교대조조감소,적분광밀도치(IOD)강저(P＜0.05),VEGF mRNA양성세포IOD치승고(P＜0.05);당귀조신생대서대뇌피질NSE mRNA양성세포수교결양조증다、IOD치증고(P＜0.05),VEGF mRNA양성세포IOD치증고(P＜0.05).결론 궁내결양가치신생대서대뇌피질신경원수손,당귀주사액대차손상유일정보호작용,기궤제가능시통과상조대뇌피질VEGF mRNA적표체이사결양배경개선.
Objective To explore the effect of intrauterine hypoxia on the neurons and the expression of VEGF mRNA in the cerebral cortex of neonatal rats, and the regulative role of angelica to them. Method Fifteen pregnant healthy SD rats were divided randomly into three groups: a control group(CG), a hypoxia group (HG) and an angelica group (AG), with each group containing 5 rats. The angelica group and hypoxia group pregnant rats were placed in a three-gas incubator from the start of pregnant 14th days after to produce the intrauterine hypoxia model of fetal rats, and one hour after they were removed from the incubators, the pregnant rats received injections of angelica and normal saline (8 ml/kg) through the caudal vein. The control group procedures were identical to the hypoxia group, but under normal, non-hypoxic conditions. After birth, four neontal rats were selected randomly from each litter and brain tissue was sampled and fixed in paraformaldehyde, embedded in paraffin and sectioned for NSE mRNA and VEGF mRNA in situ hybridization (ISH). Photos of the cerebral cortex were taken under a microscope at 400×. The photos were analyzed with IPP6.0 software. Results The quantity and integral optical density (IOD) value of positive NSE mRNA cells in the HG were obviously lower than that of the CG(P＜0.05), and the IOD value of positive VEGF mRNA cells in the HG was significantly higher than that of the CG (P＜0.05); but the quantity and IOD value of positive NSE mRNA cells in the AG were higher than that of the HG(P＜0.05), and the IOD value of positive VEGF mRNA cells in the AG was significantly higher than that of the HG(P＜0.05). Conclusion Intrauterine hypoxia could damage the neurons in the cerebral cortex of neonatal rats, and angelica injections could play a protective role to a certain degree by possibly upregulating the expression of VEGF mRNA in the cerebral cortex to ameliorate the intrauterine hypoxic environment.