CAJ | 학술논문

目的:研究切口痛大鼠脊髓背角磷酸化p38有丝分裂原活化蛋白激酶(p-p38MAPK)表达的变化和鞘内注射p38MAPK特异性抑制剂SB203580对切口痛大鼠疼痛的影响.方法:雄性SD大鼠随机分为假手术组(SH组)、切口痛组(IP组)、药物组和DMSO组.按Yaksh法施行鞘内置管.按Brennan法建立大鼠切15口疼痛模型.采用Hargreaves法(热痛觉过敏)测定热刺激缩足反射潜伏期(TWL).应用免疫组织化学法检测脊髓背角p-p38MAPK表达的变化.结果:与SH组相对应的时间点和术前值比较,IP组和DMSO组大鼠在术后2 h、3 h、6h、ld、2 d和3 d的TWL均明显缩短(P<0.05或P<0.01),但IP组和DMSO组各相对应的时间点比较无明显差异;与IP组和DMSO组相对应的时间点比较,药物组大鼠在术后2 h、3 h、6 h、1 d和2 d的TWL均明显延长(P<0.05或P<0.01).与SH组比较,IP组和DMSO组P-p38MAPK阳性细胞数增加(P<0.01);与IP组和DMSO组比较,药物组P-p38MAPK阳性细胞数降低(P<0.01或0.05),IP组和DMSO组间差异无统计学意义(P>0.05).IP组和DMSO组p-p38MAPK阳性细胞数在术后6h开始增加,1d达到高峰,然后逐渐下降,5d后逐渐恢复.结论:脊髓背角p38MAPK活化参与切口痛的形成与发展.
목적:연구절구통대서척수배각린산화p38유사분렬원활화단백격매(p-p38MAPK)표체적변화화초내주사p38MAPK특이성억제제SB203580대절구통대서동통적영향.방법:웅성SD대서수궤분위가수술조(SH조)、절구통조(IP조)、약물조화DMSO조.안Yaksh법시행초내치관.안Brennan법건립대서절15구동통모형.채용Hargreaves법(열통각과민)측정열자격축족반사잠복기(TWL).응용면역조직화학법검측척수배각p-p38MAPK표체적변화.결과:여SH조상대응적시간점화술전치비교,IP조화DMSO조대서재술후2 h、3 h、6h、ld、2 d화3 d적TWL균명현축단(P<0.05혹P<0.01),단IP조화DMSO조각상대응적시간점비교무명현차이;여IP조화DMSO조상대응적시간점비교,약물조대서재술후2 h、3 h、6 h、1 d화2 d적TWL균명현연장(P<0.05혹P<0.01).여SH조비교,IP조화DMSO조P-p38MAPK양성세포수증가(P<0.01);여IP조화DMSO조비교,약물조P-p38MAPK양성세포수강저(P<0.01혹0.05),IP조화DMSO조간차이무통계학의의(P>0.05).IP조화DMSO조p-p38MAPK양성세포수재술후6h개시증가,1d체도고봉,연후축점하강,5d후축점회복.결론:척수배각p38MAPK활화삼여절구통적형성여발전.
Objective:To investigate the change of phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK)in the spinal dorsal horn,and the effects of intrathecal administration of p38MAPK spe- cific inhibitor SB203580 on the behavior of pain in a rat model of incisional pain.Methods:Male Spra- gue-Dawley rats were randomly divided into sham group,incisional pain group,intrathecal drug group and intratheeal DMSO group.Catheter was inserted into the intratheeal space according to Yaksh's method in all rats.The incisional pain model was adopted as described by Brennan et al.Thermal withdrawal la- tency(TWL)was measured using radiant heat method.Using immunohistoehemistry method.the expres- sions of p-p38 MAPK in the spinal dorsal horn following paw incision surgery were investigated.Results: Compared to sham group and baseline of pre-incision,TWL ipsilateral to the incision in 2 h,3 h,6h, 1d,2d and 3d post-incision decreased significantly in the IP group and DMSO group(P<0.05 or P<0. 01);but compared to IP group,the change of TWL in corresponding point of time post-incision in DMSO group had no significant change:Compared to TWL in corresponding point of time after incision in IP group and DMSO group,TWL ipsilateral to the incision in 2 h,3 h,6h,1d and 2d of post-incision in- creased significantly in drug group(P<0.05 or P<0.01).Compared to sham group,the number of posi- tive ceHs of p-p38 MAPK in the spinal dorsal horn ipsilateral to the paw surgery increased significantly in IP group and DMSO group(P<0.01);Compared to IP group and DMSO group,the number of positive ceHs of p-p38 MAPK in the spinal dorsal horn ipsilateral to the paw surgery decreased significantly in drug group(P<0.05 or P<0.01);The number of positive cells between IP group and DMSO group had no significant change(P>O.05).The number of positive ceHs of p-p38MAPK in IP group and DMSO group began to increase from 6h,reached peak on 1 d,but decreased to baseline of pre-incision after 5d of post- incision.Conclusions:p38 MAPK activation in spinal dorsal hom plays important role in the formation and development of incisional pain in rats.