CAJ | 학술논문

对中国内蒙古自治区饲育马土壤环境中马红球菌的分布知之甚少;为此,在中国内蒙古通辽近郊、内蒙古南部锡林郭勒盟大草原、内蒙古东部呼伦贝尔大草原等马场共收集了108份土壤样品进行了马红球菌的检测.结果,锡林郭勒盟大草原和呼伦贝尔大草原的马红球菌分离率为25.9%～30.0%,通辽近郊土壤中分离率高达82.3%.这说明在通辽近郊马红球菌的分离菌数是草原土壤中马红球菌分离菌数的10倍.应用PCR技术检测了488株的毒力相关基因,相对分子质量分别为15 000～17 000(VapA)和20 000(VapB).所有的分离株都未检出毒力相关基因.这些无毒力分离株的质粒资料显示,各种大小的潜在质粒的发生率为13.3%～21.5%.本研究结果截然不同于我们新近对蒙古国的调查结论:马红球菌在蒙古国乌兰巴托的马群中不存在.造成这一差异的原因可能在于蒙古国的游牧生活和内蒙古的非游牧生活.
대중국내몽고자치구사육마토양배경중마홍구균적분포지지심소;위차,재중국내몽고통료근교、내몽고남부석림곽륵맹대초원、내몽고동부호륜패이대초원등마장공수집료108빈토양양품진행료마홍구균적검측.결과,석림곽륵맹대초원화호륜패이대초원적마홍구균분리솔위25.9%～30.0%,통료근교토양중분리솔고체82.3%.저설명재통료근교마홍구균적분리균수시초원토양중마홍구균분리균수적10배.응용PCR기술검측료488주적독력상관기인,상대분자질량분별위15 000～17 000(VapA)화20 000(VapB).소유적분리주도미검출독력상관기인.저사무독력분리주적질립자료현시,각충대소적잠재질립적발생솔위13.3%～21.5%.본연구결과절연불동우아문신근대몽고국적조사결론:마홍구균재몽고국오란파탁적마군중불존재.조성저일차이적원인가능재우몽고국적유목생활화내몽고적비유목생활.
Little is known about the distribution of Rhodococcus equi in the soil environment of native horses in China. One hundred and eight soil samples were collected from native-horse farms in the Hulun Beier grasslands of eastern Mongolia, the Xilin Goler grasslands of southern Mongolia, and Tongliao city in Inner Mongolia of China for investigating the distribution of R. equi in these regions. The isolation rates of R. equi from soil samples from the Hulun Beier and Xilin Goler grasslands ranged from 25.9% to 30.0%. In contrast, isolation rates from soil samples from Tongliao city was as high as 82.3% and the mean number of R. equi in soil samples from Tongliao city was 10 times more than those of samples from the grasslands. The 488 isolates were examined using PCR for the presence of genes that encode virulence-associated 15 000-17 000 antigen protein (VapA) and the 20 000 antigen protein (VapB). All isolates were negative for virulence-associated proteins. Plasmid profiles of these avirulent isolates showed that cryptic plasmids of various sizes were present with an incidence of 13.3% to 21.5%. The results of the present study contrast with those of our recent study, in which we reported that R. equi was absent from Mongolian horses in Ulaanbaatar, Mongolia. It is suggested that the difference between the results of these two studies is due to the mobile pasturing system in Mongolia and nonmobile pasturing system in Inner Mongolia.