山西医科大学学报
山西醫科大學學報
산서의과대학학보
JOURNAL OF SHANXI MEDICAL UNIVERSITY
2008年
1期
11-14
,共4页
溶血磷脂酸%神经干细胞%增殖%百日咳毒素%大鼠
溶血燐脂痠%神經榦細胞%增殖%百日咳毒素%大鼠
용혈린지산%신경간세포%증식%백일해독소%대서
lysophosphatidic acid%neural stem cell%proliferation%pertussis toxin%rats
目的 观察溶血磷脂酸(lysophosphtidic acid,LPA)对大鼠胚胎神经干细胞(neural stem cells,NSCs)的增殖的影响以及百日咳毒素(pertussis toxin,PTX)对LPA作用的影响.方法 体外培养神经干细胞,对生成的神经干细胞球进行计数显示增殖情况.结果 ①在特殊的无血清培养液中加入低浓度LPA(0.01-1.0μmol/L)后,神经干细胞球数量呈剂量依赖性增加,表明LPA对NSCs有显著的促增殖作用;②培养液中同时加入LPA和PTX后,神经干细胞球计数显示LPA引起的NSCs的增殖被抑制了98%.结论 PTX阻断了LPA对大鼠胚胎神经干细胞的促增殖作用,从而推测LPA的促增殖作用可能主要通过PTX敏感的Gi-Ras-Raf-MAPK信号转导途径实现.
目的 觀察溶血燐脂痠(lysophosphtidic acid,LPA)對大鼠胚胎神經榦細胞(neural stem cells,NSCs)的增殖的影響以及百日咳毒素(pertussis toxin,PTX)對LPA作用的影響.方法 體外培養神經榦細胞,對生成的神經榦細胞毬進行計數顯示增殖情況.結果 ①在特殊的無血清培養液中加入低濃度LPA(0.01-1.0μmol/L)後,神經榦細胞毬數量呈劑量依賴性增加,錶明LPA對NSCs有顯著的促增殖作用;②培養液中同時加入LPA和PTX後,神經榦細胞毬計數顯示LPA引起的NSCs的增殖被抑製瞭98%.結論 PTX阻斷瞭LPA對大鼠胚胎神經榦細胞的促增殖作用,從而推測LPA的促增殖作用可能主要通過PTX敏感的Gi-Ras-Raf-MAPK信號轉導途徑實現.
목적 관찰용혈린지산(lysophosphtidic acid,LPA)대대서배태신경간세포(neural stem cells,NSCs)적증식적영향이급백일해독소(pertussis toxin,PTX)대LPA작용적영향.방법 체외배양신경간세포,대생성적신경간세포구진행계수현시증식정황.결과 ①재특수적무혈청배양액중가입저농도LPA(0.01-1.0μmol/L)후,신경간세포구수량정제량의뢰성증가,표명LPA대NSCs유현저적촉증식작용;②배양액중동시가입LPA화PTX후,신경간세포구계수현시LPA인기적NSCs적증식피억제료98%.결론 PTX조단료LPA대대서배태신경간세포적촉증식작용,종이추측LPA적촉증식작용가능주요통과PTX민감적Gi-Ras-Raf-MAPK신호전도도경실현.
Objective To study the effect of lysophosphatidic acid(LPA)on the proliferation of rat embryonic neural stem cells (NSCs) in vitro and the effect of pertussis toxin (PTX) on LPA-induced promotive proliferation of NSCs. Methods NSCs were cultured in vitro, and neurospheres were counted.Results ① Lower concentrations of LPA (0.01-1.0 μmol/L) dose-dependently increased the numbers of neurospheres cultured in specific serum-free media, which indicated a significant promotive action of LPA on the proliferation of NSCs. ② After NSCs were treated by 1.0 μmol/L LPA combined with PTX, 98% of the LPA-induced proliferation was blocked. Conclusion These results suggest that the promotive effect LPA might be brought about mainly by the PTX-sensitive Gi-Ras-Raf-MAPK pathway rather than the PTX-insensitive ones.
