CAJ | 학술논문

以仙人掌为原料提取多糖产品,通过超滤浓缩和不同浓度乙醇分级沉淀,分别得到55%乙醇沉淀的多糖(OPMC I),进一步乙醇浓度增至80%沉淀的多糖 (OPMCⅡ),以及一次性80%乙醇沉淀的多糖(OPMCⅢ).比较了三种多糖对蛋白质非酶糖基化反应的终端产物(AGEs)和醛糖还原酶(AR)形成的抑制作用.结果表明,在对AGEs形成的抑制过程中,第四周时OPMC II的抑制作用较强,且强于同剂量的氨基胍;在对AR活性的抑制作用中,OPMC II在三种多糖样品中抑制作用最强,但明显低于阳性对照物依帕司他.
이선인장위원료제취다당산품,통과초려농축화불동농도을순분급침정,분별득도55%을순침정적다당(OPMC I),진일보을순농도증지80%침정적다당 (OPMCⅡ),이급일차성80%을순침정적다당(OPMCⅢ).비교료삼충다당대단백질비매당기화반응적종단산물(AGEs)화철당환원매(AR)형성적억제작용.결과표명,재대AGEs형성적억제과정중,제사주시OPMC II적억제작용교강,차강우동제량적안기고;재대AR활성적억제작용중,OPMC II재삼충다당양품중억제작용최강,단명현저우양성대조물의파사타.
Polysaccharides from Opuntia monacantha Cladode (OPMC) were extracted by distilled water and concentrated by ultrafiltration.OPMC I was obtained by precipitation OPMC extracts with 55% of ethanol solution.The supernatant was added ethanol to final concentration 80% to precipitate OPMCⅡ.OPMCⅢ was obtained by precipitation OPMC extracts with 80% of ethanol solution directly.The inhibition of three OPMC polysaccharides on the development of advanced glycated end product (AGE) of protein non-enzymaticaly glycated reaction and aldose reductase (AR) activity were investigated.OPMCⅡ showed stronger inhibition effect on AGE than aminoguanidine with the same dose at fourth week.Among OPMC Ⅰ-Ⅲ,OPMCⅡ also showed the strongest inhibition effect on AR activity,but lower than epalrestat,the positive control.