CAJ | 학술논문

背景:骨缺损修复一直是骨科领域的难题之一,近年来,组织工程化骨组织的研究为骨缺损修复提供了全新的思路和方法,而生物材料的生物相容性检测是其中重要的一个环节.目的:探讨骨髓基质细胞与聚己内酯的生物相容性.设计:对照观察.单位:新疆医科大学第一附属医院骨科.材料:实验于新疆医科大学第一附属医院骨科实验室完成.选4～8周龄健康新西兰大白兔,体质量2 kg左右.方法:①抽吸双侧股骨骨髓并混入RPMI 1640完全培养基进行细胞培养,然后进入传代培养.②进行细胞接种并分为聚己内酯组及对照组,对照组单纯接种细胞,聚己内酯组将骨髓基质细胞与聚己内酯体外复合培养,进行形态学观察、细胞增殖、蛋白质含量及酶学测定.主要观察指标:骨髓基质细胞生长及与聚乙内酯生物材料附着情况.结果:对照组细胞多向梭形细胞或多角形细胞转化.聚己内酯组骨髓基质细胞能在聚己内酯上贴附、繁殖,其生长及功能不受影响,并且聚己内酯具有一定的促细胞增殖作用.结论:聚己内酯具有良好的细胞相容性,有可能作为骨髓基质细胞的载体应用于组织工程的研究.
배경:골결손수복일직시골과영역적난제지일,근년래,조직공정화골조직적연구위골결손수복제공료전신적사로화방법,이생물재료적생물상용성검측시기중중요적일개배절.목적:탐토골수기질세포여취기내지적생물상용성.설계:대조관찰.단위:신강의과대학제일부속의원골과.재료:실험우신강의과대학제일부속의원골과실험실완성.선4～8주령건강신서란대백토,체질량2 kg좌우.방법:①추흡쌍측고골골수병혼입RPMI 1640완전배양기진행세포배양,연후진입전대배양.②진행세포접충병분위취기내지조급대조조,대조조단순접충세포,취기내지조장골수기질세포여취기내지체외복합배양,진행형태학관찰、세포증식、단백질함량급매학측정.주요관찰지표:골수기질세포생장급여취을내지생물재료부착정황.결과:대조조세포다향사형세포혹다각형세포전화.취기내지조골수기질세포능재취기내지상첩부、번식,기생장급공능불수영향,병차취기내지구유일정적촉세포증식작용.결론:취기내지구유량호적세포상용성,유가능작위골수기질세포적재체응용우조직공정적연구.
BACKGROUND: Bone defect repair is a difficulty in orthopedic field all the time, researches on the tissue engineered bone tissue have provided completely new thoughts and methods for bone defect repair, and it is an important link to detect the biocompatibility of the biomaterials.OBJECTIVE: To investigate the biocompatibility of PCL with bone marrow stromal cells (BMSCs).DESIGN: A controlled observation.SETTING: Department of Orthopaedics, the First Affiliated Hospital of Xinjiang Medical University.MATERIALS: The experiments were carried out in the orthopedic laboratory of the First Affiliated Hospital of Xinjiang Medical University. Healthy 4 to 8-week-old New Zealand rabbits of about 2 kg were used.METHODS: ① Bone marrow was extracted from bilateral femurs of the rabbits, then mixed into 10 mL RPMI1640 complete medium, and then entered the passage culture. ② The cells were inoculated and then divided into PCL group and control group, the cells were only inoculated in the control group, and the BMSCs were co-cultured with PCL in vitro in the PCL group. The morphological observed, cell proliferation, protein content and enzymological determinations were conducted.MAIN OUTCOME MEASURES: The growth and the adhesion of BMSCs on PCL biomaterials were mainly observed.RESULTS: In the control group, most of the BMSCs changed to the shape of fusiform or multi-angles. In the PCL group, the BMSCs could adhere and proliferate on PCL, and the growth and function were not affected, PCL also played a certain role in promoting the cell proliferation.CONCLUSION: PCL possess satisfactory biocompatibility, and it is possible to be used as the carrier of BMSCs in tissue engineering.