CAJ | 학술논문

目的：探讨纯中药制剂热毒清抑制肿瘤坏死因子α(TNFα)失控释放的分子机制。方法：采用细胞和分子生物学技术观察热毒清对HL-60细胞分泌炎性细胞因子——分泌型肿瘤坏死因子α(sTNFα)及肿瘤坏死因子α转换酶(TACE)mRNA表达的影响。结果：(1)1∶30稀释的热毒清能有效地控制内毒素脂多糖(LPS)刺激所引起的sTNFα的高分泌。(2)热毒清单独作用HL-60细胞时，对TACE mRNA的表达虽无明显的抑制作用，但对LPS刺激增加表达的TACE mRNA具有明显的抑制效果。结论：热毒清对LPS引起的炎性细胞因子sTNFα的分泌及对sTNFα激活的关键酶——TACE的基因表达具有双重抑制作用，提示热毒清可能是一种TACE良好的天然抑制剂。
목적：탐토순중약제제열독청억제종류배사인자α(TNFα)실공석방적분자궤제。방법：채용세포화분자생물학기술관찰열독청대HL-60세포분비염성세포인자——분비형종류배사인자α(sTNFα)급종류배사인자α전환매(TACE)mRNA표체적영향。결과：(1)1∶30희석적열독청능유효지공제내독소지다당(LPS)자격소인기적sTNFα적고분비。(2)열독청단독작용HL-60세포시，대TACE mRNA적표체수무명현적억제작용，단대LPS자격증가표체적TACE mRNA구유명현적억제효과。결론：열독청대LPS인기적염성세포인자sTNFα적분비급대sTNFα격활적관건매——TACE적기인표체구유쌍중억제작용，제시열독청가능시일충TACE량호적천연억제제。
To explore the molecular mechanism of inhibiting effects of Reduqing on the release of tumor necrosis factorα (TNFα). Methods: Using cytobiologic and molecular biologic technique to observe the effects of Reduqing on HL-60 cells in producing inflammatory cytokine secreting TNFα (sTNFα) and on mRNA expression of TNFα converting enzyme (TACE). Results: (1) Reduqing, diluted in ratio 1∶30, could effectively inhibit the increased HL-60 production of sTNF-α induced by lipopolysaccharides (LPS); (2) Although no obvious effect on TACE was shown when Reduqing was applied alone on HL-60 cells, there was evident inhibitory effect of Reduqing on TACE mRNA expression enhancement induced by LPS. Conclusion: Reduqing could have the double inhibitory effects both on sTNF-α production and on the gene expression of its key enzyme, i.e. TACE stimulated by LPS, suggesting that it might be a hopeful and excellent natural TACE inhibitor.