CAJ | 학술논문

目的评价丙烯腈对雌性大鼠有无致畸作用。方法于雌性大鼠妊娠第7～16天皮下注射丙烯腈(AN)15，25，35 mg·kg-1，常规方法检测AN的胚胎毒性和致畸作用。结果 25 mg·kg-1组死胎率、吸收胎率升高，胎鼠平均体重、体长、尾长减小，与阴性对照组比较差异有显著性(P＜0.05)；35 mg·kg-1组除窝平均活胎数减少，差异有显著性( P＜0.05)，其余各指标与阴性对照组比较差异有非常显著性(P ＜0.01)；25，35 mg·kg-1组畸胎率、母体畸胎率、活胎畸形率升高，与对照组相比差异有显著性和/或非常显著性(P＜0.05或P ＜0.01)，且有明确的剂量效应或剂量反应关系。本实验皮下注射AN染毒的致畸最低剂量为 15 mg·kg-1，致畸指数为6.3，以骨骼畸形为主，外观畸形主要为无尾、短尾等。结论丙烯腈皮下注射染毒有明显的胚胎毒性，致畸剂量小于经口染毒阈剂量，是一种弱致畸物。
목적평개병희정대자성대서유무치기작용。방법우자성대서임신제7～16천피하주사병희정(AN)15，25，35 mg·kg-1，상규방법검측AN적배태독성화치기작용。결과 25 mg·kg-1조사태솔、흡수태솔승고，태서평균체중、체장、미장감소，여음성대조조비교차이유현저성(P＜0.05)；35 mg·kg-1조제와평균활태수감소，차이유현저성( P＜0.05)，기여각지표여음성대조조비교차이유비상현저성(P ＜0.01)；25，35 mg·kg-1조기태솔、모체기태솔、활태기형솔승고，여대조조상비차이유현저성화/혹비상현저성(P＜0.05혹P ＜0.01)，차유명학적제량효응혹제량반응관계。본실험피하주사AN염독적치기최저제량위 15 mg·kg-1，치기지수위6.3，이골격기형위주，외관기형주요위무미、단미등。결론병희정피하주사염독유명현적배태독성，치기제량소우경구염독역제량，시일충약치기물。
Objective The teratogenicity of acrylonitrile(AN)on fe male rats was evaluated.Methods 15,25 and 35 mg/kg AN were administ ered by subcutaneous injection to rats on the time 7～16 d of gestation.Embryoto x icity and teratogenicity of AN were examined by a traditional teratogenic method .Results At the dosage of 25 mg/kg,AN could induce higher incidence of fetal resorption and death,and decrease the average fetal body weight and le ngth with significant difference(P＜0.05)when compared with neg ative controls.At the dosage of 35 mg/kg,AN could decrease average vital fet us p er litter with significant difference(P＜0.05),and induce chang es of other indexes with highly significant difference(P＜0.0 1).At the dosage of 25 and 35 mg/kg,AN could induce higher teras rate,maternal t eras rate and malformation rate of vital fetus with significant and/or highly s ignificant difference(P＜0.05 or P＜0.01),resp ectively.There existed a dose-effect or dose-response relationship between abo ve indexes and doses.The threshold of teratogenic effect of AN through subcutane ous injection was 15 mg/kg body weight,and the teratogenic index was 6.3.The ma jor malformation was skeletal deformity,and the major external malformations wer e tail deletion and short tail.Conclusions AN has shown remarkable embryotoxicity by subcutaneous injection.The threshold of teratogenic effect is lower than that by gavage,so AN is a weak teratogen.