CAJ | 학술논문

叶绿体转化系统，独立于传统的核转化，为植物导入外源基因提供了新途径。它有如下优点：超量表达目的基因；以定点整合方式导入外源基因从而消除了位置效应及基因沉默；具原核表达方式，能以多顺反子的形式表达多个基因；母系遗传方式可防止基因扩散；基因产物区域化并能提供适于某些产物发挥功能的小环境等。随着这项技术在越来越多的领域发挥作用，其优越性逐渐得到认同。本文着重对此技术的特点、发展及应用作一综述。
협록체전화계통，독립우전통적핵전화，위식물도입외원기인제공료신도경。타유여하우점：초량표체목적기인；이정점정합방식도입외원기인종이소제료위치효응급기인침묵；구원핵표체방식，능이다순반자적형식표체다개기인；모계유전방식가방지기인확산；기인산물구역화병능제공괄우모사산물발휘공능적소배경등。수착저항기술재월래월다적영역발휘작용，기우월성축점득도인동。본문착중대차기술적특점、발전급응용작일종술。
Chloroplast transformation, an alternative way to incorporate foreign genes into plant genome, has a number of advantages: higher transgene expression level; site-specific introduction of foreign genes which leads to the absence of gene silencing and position effect; the ability to express polycistronic messages from a single promoter; uniparental inheritance that prevents pollen transmission of foreign DNA; provision of more favorable environments for certain biochemical reactions and for accumulating large amounts of gene products. It has become a useful tool in many fields. In this paper the characteristics, recent advances and application of chloroplast transformation are reviewed.