中国人兽共患病杂志
中國人獸共患病雜誌
중국인수공환병잡지
CHINESE JOURNAL OF ZOONOSES
2001年
2期
56-58
,共3页
何似%陈润%李世清%吴护华%陈燕%徐珊%杨建娜%陈敏红
何似%陳潤%李世清%吳護華%陳燕%徐珊%楊建娜%陳敏紅
하사%진윤%리세청%오호화%진연%서산%양건나%진민홍
登革病毒%IgG抗体%IIF%DRIT
登革病毒%IgG抗體%IIF%DRIT
등혁병독%IgG항체%IIF%DRIT
目的 应用登革热快速免疫色谱测试法(Dengue Fever Rapid Immunochromatographic Test,DRIT)与间接免疫荧光法(Indirect Immunofluorescent Assay,IIF)检测登革病毒IgG抗体进行比较。方法DRIT按说明书操作,在Control线区出现紫红色线的前提下在IgG线区出现紫红色线表明IgG抗体阳性;IIF抗原片系应用本室分离的登革Ⅱ型病毒感染C6/36细胞制备,操作按常规进行。结果检查临床疑似登革热病人血清56份,IIF检测阳性30份,阳性率53.6%。DRIT检查阳性26份,阳性率46.4%,两法符合率91.07%,X2=1.50,P>0.05。检测无症状人血清68份,IIF检查阳性37份,阳性率为54.4%。DRIT检查阳性23份,阳性率为33.8%,两法符合率73.53%,X2=9.39,P<0.01。结论检测病人血清两法无显著性差别,具有操作简单,时间较短,结果易观察等优点。IIF成本较低,检测病人或无症状人群IgG抗体均较敏感,仍不失为理想的常规方法。
目的 應用登革熱快速免疫色譜測試法(Dengue Fever Rapid Immunochromatographic Test,DRIT)與間接免疫熒光法(Indirect Immunofluorescent Assay,IIF)檢測登革病毒IgG抗體進行比較。方法DRIT按說明書操作,在Control線區齣現紫紅色線的前提下在IgG線區齣現紫紅色線錶明IgG抗體暘性;IIF抗原片繫應用本室分離的登革Ⅱ型病毒感染C6/36細胞製備,操作按常規進行。結果檢查臨床疑似登革熱病人血清56份,IIF檢測暘性30份,暘性率53.6%。DRIT檢查暘性26份,暘性率46.4%,兩法符閤率91.07%,X2=1.50,P>0.05。檢測無癥狀人血清68份,IIF檢查暘性37份,暘性率為54.4%。DRIT檢查暘性23份,暘性率為33.8%,兩法符閤率73.53%,X2=9.39,P<0.01。結論檢測病人血清兩法無顯著性差彆,具有操作簡單,時間較短,結果易觀察等優點。IIF成本較低,檢測病人或無癥狀人群IgG抗體均較敏感,仍不失為理想的常規方法。
목적 응용등혁열쾌속면역색보측시법(Dengue Fever Rapid Immunochromatographic Test,DRIT)여간접면역형광법(Indirect Immunofluorescent Assay,IIF)검측등혁병독IgG항체진행비교。방법DRIT안설명서조작,재Control선구출현자홍색선적전제하재IgG선구출현자홍색선표명IgG항체양성;IIF항원편계응용본실분리적등혁Ⅱ형병독감염C6/36세포제비,조작안상규진행。결과검사림상의사등혁열병인혈청56빈,IIF검측양성30빈,양성솔53.6%。DRIT검사양성26빈,양성솔46.4%,량법부합솔91.07%,X2=1.50,P>0.05。검측무증상인혈청68빈,IIF검사양성37빈,양성솔위54.4%。DRIT검사양성23빈,양성솔위33.8%,량법부합솔73.53%,X2=9.39,P<0.01。결론검측병인혈청량법무현저성차별,구유조작간단,시간교단,결과역관찰등우점。IIF성본교저,검측병인혹무증상인군IgG항체균교민감,잉불실위이상적상규방법。
Aim To compare IgG antibody tests for diagnosis of dengus fever with Denger Fever IgM and IgG Rapid immunochromatographic Test(DRIT, Panbio, Australia) and Indirect Immunofluorescent Assay(IIF). Method DRIT was followed the kit's direction, and the IgG positive result(only IgG was compared in the sutdy) was determined only appearing two amaranth lines,one is in control region, another is in IgG region. Antigen slide in IIF was prepared with dengus virus type 2 isolated in the laboratory and it was performed routinely. Results 56 sera were collected from the suspected patients with dengus fever. Among these specimens, IIF positive rate was 53.6 % (30/56), and DRIT was 46.4 % (26/56), there were no significant difference between the two tests. In comparison with IIF however, when testng 68 sera collected from the asymptom population, positive rate was 54% (37/68)for IIF, and 33.8 % (23/68) for DRIT, there existed significant difference between the two methods( X2= 9.39, P < 001). Conclusion There is no significant difference bwtween IIF and DRIT in test the IgG antibody of the patents. DRIT is a simpl, rapid, and easy method to the determination, but IIF is a ideal routine technique for use in both of the patients and the asymptom population since it's rather economic, and more sensitive.