临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2001年
2期
149-151
,共3页
肝素%肺%成纤维细胞%胶原
肝素%肺%成纖維細胞%膠原
간소%폐%성섬유세포%효원
目的：探讨不同剂量的肝素对胎鼠肺成纤维细胞（LFb）Ⅰ、Ⅲ型胶原mRNA表达的影响。方法：采用Northern印迹杂交方法检测Ⅰ、Ⅲ型胶原mRNA的表达。结果：浓度为0.1 mg/L、1 mg/L、10 mg/L的肝素对Ⅰ、Ⅲ型胶原mRNA的表达的影响不明显；100 mg/L的肝素则抑制Ⅰ、Ⅲ型胶原mRNA的表达。结论：Ⅰ、Ⅲ型胶原mRNA的表达与肝素剂量有一定的关系。
目的：探討不同劑量的肝素對胎鼠肺成纖維細胞（LFb）Ⅰ、Ⅲ型膠原mRNA錶達的影響。方法：採用Northern印跡雜交方法檢測Ⅰ、Ⅲ型膠原mRNA的錶達。結果：濃度為0.1 mg/L、1 mg/L、10 mg/L的肝素對Ⅰ、Ⅲ型膠原mRNA的錶達的影響不明顯；100 mg/L的肝素則抑製Ⅰ、Ⅲ型膠原mRNA的錶達。結論：Ⅰ、Ⅲ型膠原mRNA的錶達與肝素劑量有一定的關繫。
목적：탐토불동제량적간소대태서폐성섬유세포（LFb）Ⅰ、Ⅲ형효원mRNA표체적영향。방법：채용Northern인적잡교방법검측Ⅰ、Ⅲ형효원mRNA적표체。결과：농도위0.1 mg/L、1 mg/L、10 mg/L적간소대Ⅰ、Ⅲ형효원mRNA적표체적영향불명현；100 mg/L적간소칙억제Ⅰ、Ⅲ형효원mRNA적표체。결론：Ⅰ、Ⅲ형효원mRNA적표체여간소제량유일정적관계。
Purpose To study the effect of heparin on expression of type collagen Ⅰ,ⅢmRNA in cultured fetal rat lung fibroblast (LFb). Methods By using Northern blot method and immage analysis,the expression of collagenⅠ, Ⅲ mRNA were quantitatively analysed in the fibroblasts treated with different concentration heparin (0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L)separately. Results Heparin treatment at 0.1 mg/L,1 mg/L, 10 mg/L and did not influence the expression of collagen Ⅰ,Ⅲ mRNA，but the expression of collagen Ⅰ,Ⅲ mRNA were inhibited at 100 mg/L. Conclusion Expression of collagen Ⅰ, ⅢmRNA is related to the concentration of heparin.
