CAJ | 학술논문

建立了一个测定过氧亚硝基阴离子（ONOO－）化学发光的改进体系，测试了某些抗氧化剂清除ONOO－的作用，其体系的组成和启动发光的程序如下：向pH 10.5碳酸缓冲液配的0.01 mol/L浓度NaN3溶液通O3 30 s，取其800 μl原位注入含有100 μl水配样品和100 μl的0.001 mol/L鲁米诺溶液中，启动化学发光（chemiluminescence, CL），立即测定每6秒的脉冲数（CP6S），连续测定10～30次.根据实际需要，选其某一次的CL强度作为评判指标，对比抗氧化剂的活性.该发光体系灵敏、简便、且较稳定，最低可检测限为8.74 μmol/L的ONOO－量，线性范围为8.74～74.04 μmol/L.批内变异系数3.35%（n=10），批间变异系数5.52%（n=10）.测得维生素C(Vit.C)、茶多酚（EGCG）、原花菁素、硫脲皆有抑制CL，即清除ONOO－的作用.
건립료일개측정과양아초기음리자（ONOO－）화학발광적개진체계，측시료모사항양화제청제ONOO－적작용，기체계적조성화계동발광적정서여하：향pH 10.5탄산완충액배적0.01 mol/L농도NaN3용액통O3 30 s，취기800 μl원위주입함유100 μl수배양품화100 μl적0.001 mol/L로미낙용액중，계동화학발광（chemiluminescence, CL），립즉측정매6초적맥충수（CP6S），련속측정10～30차.근거실제수요，선기모일차적CL강도작위평판지표，대비항양화제적활성.해발광체계령민、간편、차교은정，최저가검측한위8.74 μmol/L적ONOO－량，선성범위위8.74～74.04 μmol/L.비내변이계수3.35%（n=10），비간변이계수5.52%（n=10）.측득유생소C(Vit.C)、다다분（EGCG）、원화정소、류뇨개유억제CL，즉청제ONOO－적작용.
An improvement of chemiluminescent system for determination of peroxynitrite anion (ONOO－) has been made. In this system, the effect of some antioxidants for scavenging ONOO－ was tested. The constitute of this system and the program of starting were followed as: The ozone (O3) was bubbled through a glass-frit into 10 ml 0.01 mol/L solution of sodium azide in CB(pH 10.5) to generate ONOO－. The 800 μl ozonized solution of azide was injected into a glass tude in situ which contains 100 μl sample and 100 μl luminol solutions to initiate chemiluminescence (CL). The pluses / 6 seconds (CP6S) were determined immediately and continually for 10～30 times. A certain CL intensity (CP6S) was chosen as evaluation index to compare the activity of antioxidants. This chemiluminescent system is sensible, simple and stable. The determination limit was 8.74 μmol/L ONOO－. The linear rang was 8.74～74.04 μmol/L ONOO－. The intra batch and inter batch variation coefficient (CV%) of the analysis were 3.35%(n=10) and 5.52%(n=10) respectively. It was tested that Vit.C, teapolyphenol, procyanidin and thiourea all have effects on scavenging ONOO－.