上海医学
上海醫學
상해의학
SHANGHAI MEDICAL JOURNAL
2001年
3期
169-172
,共4页
狼疮性肾炎%尿激酶型纤溶酶原激活物(u-PA)%Ⅰ型纤溶酶原激活物抑制因子(PAI-1)
狼瘡性腎炎%尿激酶型纖溶酶原激活物(u-PA)%Ⅰ型纖溶酶原激活物抑製因子(PAI-1)
랑창성신염%뇨격매형섬용매원격활물(u-PA)%Ⅰ형섬용매원격활물억제인자(PAI-1)
目的了解尿激酶型纤溶酶原激活物(u-PA)及其Ⅰ型抑制因子(PAI-1)在狼疮性肾炎(LN)肾小球病变中的表达及其意义。方法对50例不同类型的LN和10例原发性肾小球轻微病变的肾活检组织，采用免疫组化及图象分析法比较观察了u-PA和PAI-1在肾小球的表达强度，并与肾小球增殖细胞核抗原(PCNA)阳性细胞数和Ⅳ型胶原染色强度作相关分析。结果u-PA和PAI-1在各型LN病变肾小球中的表达，均比肾小球轻微病变明显增强(P<0.05或<0.01)，并以LN-IV型为最明显。PAI-1的染色反应强于同组的u-PA，两者有显著差异(P<0.05)。肾小球u-PA和PAI-1的表达均与PCNA阳性细胞核数呈正相关(P<0-05)。而且PAI-1的表达还与肾小球内IV型胶原沉积呈正相关(P<0.05)。结论狼疮性肾炎中u-PA和PAI-1的异常表达与肾小球的病变程度和病理类型相关，并可能是影响狼疮性肾炎发生发展的重要因素。
目的瞭解尿激酶型纖溶酶原激活物(u-PA)及其Ⅰ型抑製因子(PAI-1)在狼瘡性腎炎(LN)腎小毬病變中的錶達及其意義。方法對50例不同類型的LN和10例原髮性腎小毬輕微病變的腎活檢組織，採用免疫組化及圖象分析法比較觀察瞭u-PA和PAI-1在腎小毬的錶達彊度，併與腎小毬增殖細胞覈抗原(PCNA)暘性細胞數和Ⅳ型膠原染色彊度作相關分析。結果u-PA和PAI-1在各型LN病變腎小毬中的錶達，均比腎小毬輕微病變明顯增彊(P<0.05或<0.01)，併以LN-IV型為最明顯。PAI-1的染色反應彊于同組的u-PA，兩者有顯著差異(P<0.05)。腎小毬u-PA和PAI-1的錶達均與PCNA暘性細胞覈數呈正相關(P<0-05)。而且PAI-1的錶達還與腎小毬內IV型膠原沉積呈正相關(P<0.05)。結論狼瘡性腎炎中u-PA和PAI-1的異常錶達與腎小毬的病變程度和病理類型相關，併可能是影響狼瘡性腎炎髮生髮展的重要因素。
목적료해뇨격매형섬용매원격활물(u-PA)급기Ⅰ형억제인자(PAI-1)재랑창성신염(LN)신소구병변중적표체급기의의。방법대50례불동류형적LN화10례원발성신소구경미병변적신활검조직，채용면역조화급도상분석법비교관찰료u-PA화PAI-1재신소구적표체강도，병여신소구증식세포핵항원(PCNA)양성세포수화Ⅳ형효원염색강도작상관분석。결과u-PA화PAI-1재각형LN병변신소구중적표체，균비신소구경미병변명현증강(P<0.05혹<0.01)，병이LN-IV형위최명현。PAI-1적염색반응강우동조적u-PA，량자유현저차이(P<0.05)。신소구u-PA화PAI-1적표체균여PCNA양성세포핵수정정상관(P<0-05)。이차PAI-1적표체환여신소구내IV형효원침적정정상관(P<0.05)。결론랑창성신염중u-PA화PAI-1적이상표체여신소구적병변정도화병리류형상관，병가능시영향랑창성신염발생발전적중요인소。
Objective To investigate the expression and significance of urokinase-type plasminogen activator (uPA) and type Ⅰ plasminogen activator inhibitor (PAI-1) in human lupus nephritis (LN). Method Immunohistochemistry and imaging analysis were used for detecting the intensity of expression of u-PA and PAI-1 in renal biopsied tissue of 50 cases with various types of LN and 10 cases with minor glomerular lesions (control group), the results were analyzed correlatively with PCNA cells and type IV collagen. Results The levels of u-PA and PAI-1 expression were different in various type of LN , in which, both were significantly higher than those of the minor glomerular lesion group (P＜0.05), and the highest was LN-IV group. The staining intensity of PAI-1 expression was significantly stronger than that of u-PA in various type of LN (P ＜0.05), the increased expression of u-PA and PAI-1 were positively correlated with increased number of PCNA + cell (P ＜0.05), while the expression of PAI-1 was also closely related to increased deposition of type IV collagen in the glomeruli ( P ＜0.05). Conclusion The abnormal expression of u-PA and PAI-1 were associated with the extent of injury and types of LN and may be one of important factor in contributing to the progression and inimry in LN. (Shanghai Med J, 2001,24:169)
