CAJ | 학술논문

目的探讨酪氨酸羟化酶(TH)和GTP环水解酶-1（GCH）基因修饰的永生化成纤维细胞的可行性和表达能力，为其混合移植治疗帕金森病(PD)作准备。方法 SV40大T抗原转化成纤维细胞使其永生化。构建TH和GCH基因真核表达质粒并转染永生化成纤维细胞，利用免疫组化和RT-PCR检测其在体内外的表达。结果 TH和GCH基因修饰的永生化成纤维细胞在体外可长期传代培养且可有效表达，脑内移植后TH基因至少可表达8周以上。结论 TH基因和GCH修饰的永生化成纤维细胞在PD治疗方面具有较好的应用前景。
목적탐토락안산간화매(TH)화GTP배수해매-1（GCH）기인수식적영생화성섬유세포적가행성화표체능력，위기혼합이식치료파금삼병(PD)작준비。방법 SV40대T항원전화성섬유세포사기영생화。구건TH화GCH기인진핵표체질립병전염영생화성섬유세포，이용면역조화화RT-PCR검측기재체내외적표체。결과 TH화GCH기인수식적영생화성섬유세포재체외가장기전대배양차가유효표체，뇌내이식후TH기인지소가표체8주이상。결론 TH기인화GCH수식적영생화성섬유세포재PD치료방면구유교호적응용전경。
Objective　To explore the expression of tyrosine hydroxylase(TH) and GTP cyclohydrolase-1 (GCH) gene in genetically modified immortalized fibroblasts for potential use in the treatment of Parkinson's disease (PD). Methods　Rat fibroblasts were immortalized by SV40 large antigen transformation. Eukaryotic plasmid vector encoding TH gene or GCH gene was constructed and introduced into the fibroblasts respectively, and immunohistochemistry or RT-PCR was performed to identify the exogeneous gene expression in vitro and in vivo. Results　Immortalized fibroblasts genetically modified by TH gene and GCH gene could survive long-termly and express the exogenous gene in vitro. When the gene modified fibroblasts were intracerebrally transplanted, TH gene was expressed for at least 8 weeks without tumor-forming or immune-repelling phenomena. Conclusion　Immortalized fibrblasts genetically modified by TH and GCH gene may be developed as a useful therapy for PD.