CAJ | 학술논문

【目的】研究抗人食管癌单克隆抗体G9(McAbG9)在荷瘤裸鼠体内对食管癌组织的定位作用。【方法】采用氯胺-T法制备标记物125I-G9，纯化后腹腔注射，分别于24、48、72 h取多种器官/组织测放射性，计算每克组织摄取放射性的百分比（%ID/g）及肿瘤与非瘤组织的比T/NT，并取72 h的肿瘤组织进行放射自显影分析。【结果】 125　I-G9肿瘤组织的放射性均较其它器官/组织高（血液除外），48 h组最高，T/NT值在2～7之间；放射自显影显示肿瘤组织有大量放射性浓集，肿瘤边缘较中心部位明显。【结论】 125　I-G9能在荷瘤裸鼠体内定位于食管癌部位，单抗G9具有很好的导向作用。
【목적】 연구항인식관암단극륭항체G9(McAbG9)재하류라서 체내대식관암조직적정위작용。【방법】 채용록알-T법제비표기물125I-G9，순화 후복강주사，분별우24、48、72 h취다충기관/조직측방사성，계산매극조직섭취방사성적 백분비（%ID/g）급종류여비류조직적비T/NT，병취72 h적종류조직진행방사자현영분석。【결과】 125　I-G9종류조직적방사성균교기타기관/조직고（혈액제외），48 h조 최고，T/NT치재2～7지간；방사자현영현시종류조직유대량방사성농집，종류변연교중심부 위명현。【결론】 125　I-G9능재하류라서체내정위우식관암부위，단항G9구유흔호적도향작용。
【Objective】 To study the location effect of the Mc Ab G9 to human esophageal carcinoma in tumor bearing nude mice. 【Methods】 125　I-G9 was prepared by Chloramine-T method. The distribution of 125 　 I-G9 was detected at different time (24, 48, and 72 h) after peritoneal injecti on. The percentage of the injected dose per gram of tissue(%ID/g) and the ratio of Tumor/non-Tumor were calculated. 【Results】 The distribution of 125　 I -G9 in tumor at the third day was showed by autoradiography obviously higher th an in other organ/tissue (except blood) and the highest is at the 48 h. The T/NT values are 2-7. The autoradiography indicated that radioactivity concentrated in tumor tissue. The concentration in tumor edge is more obvious than in the ce nter. 【Conclusion】 125　I-G9 has a considerable targeting activity and can well locate in esophageal carcinoma tissue in tumor bearing nude mice.