CAJ | 학술논문

目的考察慢性粒细胞白血病(CML)树突状细胞(DC)刺激自体骨髓T细胞,清除白血病细胞的作用。方法采集3例CML病人(2例慢性期和1例急变期)外周血,分离单个核细胞(PBMNC),经塑料贴壁后在含自体血浆、重组人白介素4(rhIL-4)、重组人粒单集落刺激因子(rhGM-CSF)和重组人肿瘤坏死因子(rhTNFα)的RPMI-1640培养液,37℃,5%CO2孵箱培养10天,或用免疫磁珠从PBMNC分离DC,在含自体血浆、rhGM-CSF和rhTNFα的RPMI1640培养液培养3天,收获的细胞即DC加到含rhIL-2的Dexter骨髓长期培养体系,置37℃,5%CO2孵箱培养7天,观察培养前后骨髓细胞的免疫表型和P210阳性细胞比例。结果从CML病人外周血获得的DC大多数表达P210。在含rhIL-2的Dexter体系加入DC能进一步增加体系中的T细胞数。在2例慢性期病人体系中的P210阳性细胞减少,但在急性期病例,体系中P210细胞明显增加。结论 CML病人的DC表达P210,能刺激自体骨髓中T细胞增生,在慢性期病人,这些自体DC活化的骨髓T细胞有清除白血病细胞的作用。
목적고찰만성립세포백혈병(CML)수돌상세포(DC)자격자체골수T세포,청제백혈병세포적작용。방법채집3례CML병인(2례만성기화1례급변기)외주혈,분리단개핵세포(PBMNC),경소료첩벽후재함자체혈장、중조인백개소4(rhIL-4)、중조인립단집락자격인자(rhGM-CSF)화중조인종류배사인자(rhTNFα)적RPMI-1640배양액,37℃,5%CO2부상배양10천,혹용면역자주종PBMNC분리DC,재함자체혈장、rhGM-CSF화rhTNFα적RPMI1640배양액배양3천,수획적세포즉DC가도함rhIL-2적Dexter골수장기배양체계,치37℃,5%CO2부상배양7천,관찰배양전후골수세포적면역표형화P210양성세포비례。결과종CML병인외주혈획득적DC대다수표체P210。재함rhIL-2적Dexter체계가입DC능진일보증가체계중적T세포수。재2례만성기병인체계중적P210양성세포감소,단재급성기병례,체계중P210세포명현증가。결론 CML병인적DC표체P210,능자격자체골수중T세포증생,재만성기병인,저사자체DC활화적골수T세포유청제백혈병세포적작용。
Objective To investigate the effect of autologous dendritic cells(DC) to activate T lymphocytes and purify bone marrow of chronic myelogenous leukemia(CML). Methods Peripheral blood was collected from 3 cases of CML(2 in chronic phase and the other with acute transformation) and peripheral blood mononuclear cells(PBMNC) were seperated. DC were obtained by means of either culturing plastic-adherent PBMNC in RPMI 1640 containing autologous plasma, rhIL-4, rhGM-CSF and rhTNFα for 10 days or selecting negatively with immunological magnetic beads and culturing the selected cells in RPMI 1640 containing autologous plasma, rhGM-CSF and rhTNFα for 3 days. The obtained DC were added to the Dexter bone marrow long-term culture system with rhIL-2 and the culture was maintained for 7 days. Immunological phenotypes and percentage of P210 positive cells were analysed before and after the culture. Results To obtain DC from the peripheral blood of CML patients, the number were comparable to that of healthy volunteer, most of which express P210. Adding DC to Dexter system containing rhIL-2 appeard to further increase T cells in all three cases. In both cases with chronic phase, the percentage of P210 cells appeared to decrease. However, significant increase of P210 positive cells were found in the case with acute transformation. Conclusion Dendritic cells from CML patients do express P210 and are able to stimulate proliferation of T lymphocytes in the bone marrow. In chronic phase patients, DC-activated T lymphocytes appear to have some effects in purification of leukemic marrow.