CAJ | 학술논문

在急性、慢性在体大鼠及离体脑片癫癇模型上观察EEG、深部电图、神经元网络及单个神经元的电活动,研究中枢同步化电振荡行为在癫癇发生中的重要作用.实验结果表明:离体脑片(n=40)上强直电刺激海马Schaeffer侧枝,32.5% CA1神经元全细胞记录呈现3～100 Hz膜电位振荡,这种电振荡能促进细胞外场电位癫癇活动形式的转化.急性癫癇模型上(n=10,in vivo),反复强直电刺激海马或中部颞叶新皮质可诱发海马区4～7 Hz和中部颞叶新皮质20 Hz(n=11)深部电图电振荡.慢性强直电刺激大鼠背侧海马(n=12)或中部颞叶新皮质(n=11),7～8 d后出现这些脑区和EEG电振荡活动的频率及幅度的增加.巨大EEG节律性电振荡活动成为电图和癫癇行为性发作的先兆.说明过度激活海马-内嗅皮质-颞叶新皮质产生的同步化电振荡可能是颞叶癫癇发生的重要原因之一.
재급성、만성재체대서급리체뇌편전간모형상관찰EEG、심부전도、신경원망락급단개신경원적전활동,연구중추동보화전진탕행위재전간발생중적중요작용.실험결과표명:리체뇌편(n=40)상강직전자격해마Schaeffer측지,32.5% CA1신경원전세포기록정현3～100 Hz막전위진탕,저충전진탕능촉진세포외장전위전간활동형식적전화.급성전간모형상(n=10,in vivo),반복강직전자격해마혹중부섭협신피질가유발해마구4～7 Hz화중부섭협신피질20 Hz(n=11)심부전도전진탕.만성강직전자격대서배측해마(n=12)혹중부섭협신피질(n=11),7～8 d후출현저사뇌구화EEG전진탕활동적빈솔급폭도적증가.거대EEG절률성전진탕활동성위전도화전간행위성발작적선조.설명과도격활해마-내후피질-섭협신피질산생적동보화전진탕가능시섭협전간발생적중요원인지일.
To study the functional significance of oscillations in epileptogenesis we observed the relations between synchronized oscillations and epileptic events at the level of EEG,depth electrographes,local neural networks and single neuron in animal experiments in vivo or in vitro.Works were done on three models of epilepsy:kindling model on 400 μm thick horizontal rat brain slices containing the hippocampus(HPC) and entorhinal cortex(EC),acute and chronic epilepsy model in vivo.Tetanus(60 Hz,2 s,0.4～0.6 mA)was used to induce epilepsy events.The results showed that ①In 32.5%(13/40) brain slices both intracellular and extracellular recordings displayed membrane potential oscillations in CA1 neuron at 3～100 Hz induced by electric stimulus into Schaeffer collaterals.These oscillations at 7 Hz were related to synchronous field epileptiform events and promote its conversion.②In acute epilepsy model(n=10) spontaneous oscillations in the HPC at 4～7 Hz or in the medial temporal neocortex (MTNC) at 20 Hz were activated by tetanus into the MTNC or the HPC,respectively.The increased field oscillations could transfer into associated epilepsy events.③In the epileptic rats,the amplitude and frequency of spontaneous oscillations in EEG and depth electrograph were enhanced by chronically stimulating the dorsal HPC(n=12)and MTNC(n=11)respectively.Robust EEG rhythmic oscillations became prediction for behavioral and electrographic seizures in kindled rats.Tetanus-induced synchronous oscillations in the HPC-MTNC-cerebral cortex neural pathway could elicit the generation and development of epilepsy.