CAJ | 학술논문

目的研究SOD复合酶对兔细菌性角膜溃疡的保护作用及其作用机理。方法在体外向接种了绿脓杆菌的培养基上分别加入丁胺卡那霉素、SOD复合酶等，观察抑菌圈的直径。建立兔眼绿脓杆菌性角膜溃疡模型，给予丁胺卡那霉素治疗，加用 SOD复合酶作为辅助治疗，而灭活 SOD复合酶作为对照，观察临床变化，并进行病理学检查和丙二醛测定。结果体外实验证实 SOD复合酶没有抗菌作用。动物实验中，加用 SOD复合酶的兔眼，临床表现及病理改变较轻，丙二醛含量较低。结论兔眼细菌性角膜溃疡中，自由基及脂质过氧化参与了对组织的损伤，而 SOD复合酶通过抗自由基及脂质过氧化而消除这种损伤，对角膜有一定的保护作用。
목적연구SOD복합매대토세균성각막궤양적보호작용급기작용궤리。방법재체외향접충료록농간균적배양기상분별가입정알잡나매소、SOD복합매등，관찰억균권적직경。건립토안록농간균성각막궤양모형，급여정알잡나매소치료，가용 SOD복합매작위보조치료，이멸활 SOD복합매작위대조，관찰림상변화，병진행병이학검사화병이철측정。결과체외실험증실 SOD복합매몰유항균작용。동물실험중，가용 SOD복합매적토안，림상표현급병리개변교경，병이철함량교저。결론토안세균성각막궤양중，자유기급지질과양화삼여료대조직적손상，이 SOD복합매통과항자유기급지질과양화이소제저충손상，대각막유일정적보호작용。
Objective　To observe the effect of superoxide dismutase(SOD)-compound on bacterial ulceration in rabbit's eyes and explore its mechanism.Methods　Amikacin and SOD-compound etc.were added to agar inoculating PS.Pyocyaneus and the diameter of inhabiting circles were measured.The rabbit model of bacterial corneal ulcer was created and then treated with amikacin.SOD-compound was used as an adjunctive treatment while inactive SOD-compound as a control.The clinical manefestation and its pathological change were observed and the amlonyldialdehyde (MDA) in the ulcer was assayed.Results　In vitro experiment indicated that SOD-compound had no anti-bacterial effect.In rabbit corneal ulcer treated with SOD-compound,the clinical manefestation and pathological change was slighter,the MDA was lower.Conclusion　In rabbit's corneal ulceration,free radical and lipid peroxidation take part in the tissue damage.SOD-compound can contradict this effect through eliminating excessive free radical and anti-lipid peroxidation.Therefore,SOD-compound has protective effect on corneal physiology and histological structure in bacterial corneal ulceration.