CAJ | 학술논문

目的是以烯醇式丙酮酸转移酶(EPT)为靶点筛选其抑制剂,以期寻找抗细菌活性样品.实验是在96孔酶标板上对来源于169个科、560个属、916种动植物2490个提取物或分离部位样品在EPT模型上进行了批量筛选.结果表明在96.15 μg/ml浓度下发现了来源于80个科、169个属、218个种的309个样品有活性,其中14个样品的IC50小于10.00 μg/ml,40个样品的IC50在10.01～30.00 μg/ml范围,83个样品的IC50在30.01～50.00 μg/ml范围,172个样品的IC50在50.01～96.15 μg/ml范围.通过以上工作我们认为以烯醇式丙酮酸转移酶为分子靶点的体外筛选方法稳定、方便、快速、微量、有效,特别适用于天然产物的抗细菌活性筛选.
목적시이희순식병동산전이매(EPT)위파점사선기억제제,이기심조항세균활성양품.실험시재96공매표판상대래원우169개과、560개속、916충동식물2490개제취물혹분리부위양품재EPT모형상진행료비량사선.결과표명재96.15 μg/ml농도하발현료래원우80개과、169개속、218개충적309개양품유활성,기중14개양품적IC50소우10.00 μg/ml,40개양품적IC50재10.01～30.00 μg/ml범위,83개양품적IC50재30.01～50.00 μg/ml범위,172개양품적IC50재50.01～96.15 μg/ml범위.통과이상공작아문인위이희순식병동산전이매위분자파점적체외사선방법은정、방편、쾌속、미량、유효,특별괄용우천연산물적항세균활성사선.
To discover inhibitors of enoylpyruvate transferase with antibacterial activity a batch of 2490 extract or fraction samples prepared from plants and animals belonging to 169 families, 560 genera and 916 species were tested on enoylpyruvate transferase bioassay in 96-well microtiterplates. Finally 309 samples, which belong to 80 families, 169 genera and 218 species, showed inhibitory activity at 96.15 μg/ml, in which 14 samples showed IC50 at=<10.00 μg/ml, 40 samples showed IC50 at 10.01-30.00 μg/ml, 83 samples showed IC50 at 30.01-50.00 μg/ml and 172 samples showed IC50 at 50.01-96.15 μg/ml.It is indicated that this in-vitro bioassay is convenient, stable, rapid, sensitive and effective in searching for antibacterial activity samples from natural sources.