CAJ | 학술논문

体外观察干扰素-γ(interferon-γ,IFN-γ)对脐血造血干/祖细胞细胞周期蛋白D(cyclin D)不同亚型表达的影响,以探讨IFN-γ造血抑制的机制.用免疫磁珠阳性分选的方法分离脐带血CD34+细胞,并进行体外扩增;用甲基纤维素半固体培养方法,观察IFN-γ对脐血造血干/祖细胞CFU-GM形成的影响;用半定量RT-PCR检测IFN-γ 对脐血造血干/祖细胞cyclinD不同亚型表达的影响.结果显示IFN-γ可以显著抑制脐血CFU-GM的形成,并可以从mRNA水平抑制cyclin D2和Cyclin D3亚型的表达.由此得出结论,IFN-γ可以显著抑制cyclin D的表达,这可能是IFN-γ介导造血抑制的一种机制.
체외관찰간우소-γ(interferon-γ,IFN-γ)대제혈조혈간/조세포세포주기단백D(cyclin D)불동아형표체적영향,이탐토IFN-γ조혈억제적궤제.용면역자주양성분선적방법분리제대혈CD34+세포,병진행체외확증;용갑기섬유소반고체배양방법,관찰IFN-γ대제혈조혈간/조세포CFU-GM형성적영향;용반정량RT-PCR검측IFN-γ 대제혈조혈간/조세포cyclinD불동아형표체적영향.결과현시IFN-γ가이현저억제제혈CFU-GM적형성,병가이종mRNA수평억제cyclin D2화Cyclin D3아형적표체.유차득출결론,IFN-γ가이현저억제cyclin D적표체,저가능시IFN-γ개도조혈억제적일충궤제.
To explore the hematopoiesis inhibition mechanisms of interferon-γ (IFN-γ), the effects of IFN-γ on the expression of the cyclin D in the umbilical cord blood hematopoietic stem/progenitor cells were observed. In the experiments the CD34+ cells were isolated from the cord blood with MIDI-MACS system; semi-solid methylcellulose culture technique was used to measure the formation of CFU-GM; the expression levels of cyclin D isoforms were assayed by semi-quantitative RT-PCR, after the hematopoietic sterm/progenitor cells were incubated with IFN -γ. The results indicated that IFN -γ could inhibit the formation of CFU-GM and down-regulate the expression of cyclin D2 and cyclin D3at the mRNA level. It is concluded that the IFN-γ could inhibit the proliferation of hematopoietic stem cells and downregulate the expression of cyclin D, that may be one mechanism underlying the hematopoietic inhibition of IFN-γ.