国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2008年
2期
226-229
,共4页
粉防己碱%翼状胬肉%成纤维细胞%增殖
粉防己堿%翼狀胬肉%成纖維細胞%增殖
분방기감%익상노육%성섬유세포%증식
tetrandrine%pterygium%fibroblasts%proliferation
目的:观察粉防己碱(tetrandrine,Tet)对体外培养人翼状胬肉成纤维细胞(human pterygium fibroblast,HPF)增殖的影响, 研究粉防己碱对翼状胬肉成纤维细胞增殖的作用,寻找辅助治疗翼状胬肉的新方法.方法:用不同浓度(0～160μmol/L)Tet作用体外培养的HPF, 观察24～96h Tet对HPF的影响.MTT法检测细胞生长抑制率, 免疫细胞化学法检测Tet干预前后HPF增殖细胞核抗原(PCNA)的表达情况. 结果:在Tet 20, 40, 80, 160μmol/L浓度作用24～72h范围内,可剂量和时间依赖性的抑制HPF增生(P<0.05).Tet干预后PCNA蛋白表达下降,当Tet的浓度在20～160μmol/L范围内能浓度依赖性地抑制细胞表达PCNA(P<0.05) 结论:Tet可显著抑制翼状胬肉成纤维细胞增生,在一定浓度和时间范围内抑制作用呈剂量与时间依赖性 .但在高浓度时(>160μmol/L)可能存在细胞毒性作用.
目的:觀察粉防己堿(tetrandrine,Tet)對體外培養人翼狀胬肉成纖維細胞(human pterygium fibroblast,HPF)增殖的影響, 研究粉防己堿對翼狀胬肉成纖維細胞增殖的作用,尋找輔助治療翼狀胬肉的新方法.方法:用不同濃度(0～160μmol/L)Tet作用體外培養的HPF, 觀察24～96h Tet對HPF的影響.MTT法檢測細胞生長抑製率, 免疫細胞化學法檢測Tet榦預前後HPF增殖細胞覈抗原(PCNA)的錶達情況. 結果:在Tet 20, 40, 80, 160μmol/L濃度作用24～72h範圍內,可劑量和時間依賴性的抑製HPF增生(P<0.05).Tet榦預後PCNA蛋白錶達下降,噹Tet的濃度在20～160μmol/L範圍內能濃度依賴性地抑製細胞錶達PCNA(P<0.05) 結論:Tet可顯著抑製翼狀胬肉成纖維細胞增生,在一定濃度和時間範圍內抑製作用呈劑量與時間依賴性 .但在高濃度時(>160μmol/L)可能存在細胞毒性作用.
목적:관찰분방기감(tetrandrine,Tet)대체외배양인익상노육성섬유세포(human pterygium fibroblast,HPF)증식적영향, 연구분방기감대익상노육성섬유세포증식적작용,심조보조치료익상노육적신방법.방법:용불동농도(0～160μmol/L)Tet작용체외배양적HPF, 관찰24～96h Tet대HPF적영향.MTT법검측세포생장억제솔, 면역세포화학법검측Tet간예전후HPF증식세포핵항원(PCNA)적표체정황. 결과:재Tet 20, 40, 80, 160μmol/L농도작용24～72h범위내,가제량화시간의뢰성적억제HPF증생(P<0.05).Tet간예후PCNA단백표체하강,당Tet적농도재20～160μmol/L범위내능농도의뢰성지억제세포표체PCNA(P<0.05) 결론:Tet가현저억제익상노육성섬유세포증생,재일정농도화시간범위내억제작용정제량여시간의뢰성 .단재고농도시(>160μmol/L)가능존재세포독성작용.
AIM: To investigate the effect of tetrandrine (Tet) on proliferation of human pterygium fibroblasts (HPF) in vitro and to search for a new method to prevent the recurrence after pterygium surgery.METHODS: With different concentrations (0 to 160μmol/L) of Tet acting on HPF cultured in vitro, the impact was observed at 24, 48, 72 hours respectively after Tet intervention. The MTT method was used to assay the biologic activities of Tet and inhibitive rate of cell growth. The expression of proliferating cell nuclear antigen (PCNA) in each group was detected by immunohistoche-mistry before and after Tet intervention.RESULTS:With different concentrations of 20, 40, 80 and 160μmol/L and acting for 24 to 72 hours, Tet could inhibit the proliferation of HPF in a dose- and time-dependent manner (P<0.05). After the intervention of Tet, the expression of PCNA protein declined. When the concentration of Tet was in the range of 20 to 160μmol/L, it was able to inhibit the expression of PCNA in a concentration-dependent manner (P<0.05).CONCLUSION:Tet could significantly inhibit the proliferation of pterygium fibroblasts, and the inhibitive action was in a dose- and time-dependent manner within a certain range of concentration. But in high concen-tration (>160μmol/L), Tet would have cytotoxity.
