湖北农业科学
湖北農業科學
호북농업과학
2009年
10期
2499-2501
,共3页
潘佑找%林强%陈香丽%胡琼%贾姗姗
潘祐找%林彊%陳香麗%鬍瓊%賈姍姍
반우조%림강%진향려%호경%가산산
放置方式%卷丹%外植体%组织培养%快速繁殖
放置方式%捲丹%外植體%組織培養%快速繁殖
방치방식%권단%외식체%조직배양%쾌속번식
putting way%L. lancifolium%explant%tissue culture%rapid propagation
以不同放置方式的卷丹鳞片为外植体,进行组织培养已成功获得再生植株,并已建立快速无性繁殖体系.3种不同放置方式分别为:鳞片内侧向上平放、鳞片竖直插入、鳞片内侧向下平放.以MS培养基+不同的激素配比进行培养,结果表明,不同放置方式的鳞片分化能力由强到弱依次为:鳞片内侧向上平放>鳞片竖直插入>鳞片内侧向下平放.
以不同放置方式的捲丹鱗片為外植體,進行組織培養已成功穫得再生植株,併已建立快速無性繁殖體繫.3種不同放置方式分彆為:鱗片內側嚮上平放、鱗片豎直插入、鱗片內側嚮下平放.以MS培養基+不同的激素配比進行培養,結果錶明,不同放置方式的鱗片分化能力由彊到弱依次為:鱗片內側嚮上平放>鱗片豎直插入>鱗片內側嚮下平放.
이불동방치방식적권단린편위외식체,진행조직배양이성공획득재생식주,병이건립쾌속무성번식체계.3충불동방치방식분별위:린편내측향상평방、린편수직삽입、린편내측향하평방.이MS배양기+불동적격소배비진행배양,결과표명,불동방치방식적린편분화능력유강도약의차위:린편내측향상평방>린편수직삽입>린편내측향하평방.
The clonal propagations of L. lancifolium were developed through tissue culture of bud scales in vitro. Three ways of putting the explant on the culture media was the inner side of the scale upwards,the scale insert upright and the inner side of the scale downwards. The explant was cultivated on MS medium with different hormone. The result showed that the ability of the explant differentiation from strong to weak was the inner side of the scale upwards > the scale insert upright > the inner side of the scale downwards.