CAJ | 학술논문

将以原油驯化所得的纯培养微生物菌株作为模板,采用PCR(聚合酶链式反应)法,用扩增细菌16S rDNA的一对通用引物(8f,1492r)对模板扩增,并用限制性内切酶RsaI和MspI对PCR产物进行ARDRA多态性分析.聚类去除重复菌株后60株菌株可分为11种不同分类操作单元(OTU),同时得出每个分类操作单元的数量.实验结果表明,此方法可快速去除重复菌株并反映出菌株间的系统进化关系;同时实验数据可构建成库,使后续分离菌株的筛选工作只需比对数据即可完成.
장이원유순화소득적순배양미생물균주작위모판,채용PCR(취합매련식반응)법,용확증세균16S rDNA적일대통용인물(8f,1492r)대모판확증,병용한제성내절매RsaI화MspI대PCR산물진행ARDRA다태성분석.취류거제중복균주후60주균주가분위11충불동분류조작단원(OTU),동시득출매개분류조작단원적수량.실험결과표명,차방법가쾌속거제중복균주병반영출균주간적계통진화관계;동시실험수거가구건성고,사후속분리균주적사선공작지수비대수거즉가완성.
In this study, pure culture microbial strains, which were acclimated by crude oil, were used as templates. A way of screening and identifying of oil-degrading bacteria was established, using PCR technique (premier 8f,1492r) and ARDRA patterns which used enzyme digestion ( Rsal and Mspl) of cloned 16S rDNA gene sequences. The results showed that 60 strains could be divided into 11 operational axonomic unit ( OTU). And the ecological characteristics of these cloning communities could be analyzed by this method. The experimen-tal data can be built into a database which will make the further research more efficient.