CAJ | 학술논문

目的体外实验初步探讨叶下珠醇提物对人胃癌细胞MKN28生长的影响.方法通过集落形成试验观察叶下珠醇提物对人胃癌细胞MKN28集落形成的影响;将不同浓度叶下珠醇提物作用于MKN28细胞,倒置显微镜下观察细胞形态变化,MTT法测定叶下珠醇提物对MKN28细胞的增殖抑制率;流式细胞仪检测叶下珠醇提物作用48 h后MKN28细胞的凋亡情况.结果在2mg/mL和1 mg/mL2个浓度下叶下珠醇提物对MKN28胃癌细胞集落形成具有不同程度的抑制作用,浓度大者.抑制作用更强.MTT法测定细胞增殖抑制率结果表明,叶下珠醇提物对MKN28细胞增殖有抑制作用,其抑制效应具有浓度和时间依赖性.流式细胞检测提示叶下珠醇提物能诱导人胃癌细胞MKN28发生凋亡.结论叶下珠醇提物能抑制人胃癌细胞MKN28增殖,凋亡是其作用机制之一.
목적 체외실험초보탐토협하주순제물대인위암세포MKN28생장적영향.방법 통과집락형성시험관찰협하주순제물대인위암세포MKN28집락형성적영향;장불동농도협하주순제물작용우MKN28세포,도치현미경하관찰세포형태변화,MTT법측정협하주순제물대MKN28세포적증식억제솔;류식세포의검측협하주순제물작용48 h후MKN28세포적조망정황.결과 재2mg/mL화1 mg/mL2개농도하협하주순제물대MKN28위암세포집락형성구유불동정도적억제작용,농도대자.억제작용경강.MTT법측정세포증식억제솔결과표명,협하주순제물대MKN28세포증식유억제작용,기억제효응구유농도화시간의뢰성.류식세포검측제시협하주순제물능유도인위암세포MKN28발생조망.결론 협하주순제물능억제인위암세포MKN28증식,조망시기작용궤제지일.
OBJECTIVE To carry out in vitro experiment to investigate into the effect of phyllanthus urinaria alcohol extract on the growth of human stomach cancer cell MKN28. METHOD Colony formation test was adopted to observe the effect of MKN28, MTT method was used to determine its multiplication- inhibiting rate, and flow cytometry was used to detect MKN28 cell apoptosis 48 hours after phyllanthus urinaria alcohol extract took, effect. RESULT Under two different concentrations (2 mg/mL and 1 mg/mL), the extract had different degrees of inhibiting effect on MKN28 colony formation, and the higher the concentration, the better the inhibiting effect. MTT method determination showed that the extract had inhibiting effect on the multiplication of MKN28 cells and the inhibiting effect was dependent on concentration and time. The result of flow cytometry suggested that the extract could induce MKN28 apoptosis. CONCLUSION The alcohol extract of phyllanthus ruinaria can inhibit the growth of human stomach cell MKN28, and apoptosis is one of its mechanisms.