CAJ | 학술논문

采用聚偏氟乙烯中空纤维微孔膜,考察结晶过程中蛋白质溶液和洗脱液流速的改变以及不同膜面积对溶剂跨膜通量及木瓜蛋白酶晶体尺寸的影响.结果表明:在以pH值为4.7的0.1 mol/L醋酸钠为缓冲溶液、1%的(NH_4)_3PO_4为沉淀剂以及25%的MgCl_2溶液作为洗脱液的木瓜蛋白酶动态膜结晶中,当有效膜面积为62.83 cm~2,蛋白质溶液和洗脱液流速分别控制在4 744 μm/s和1.37×10~4μm/s的高流速时膜组件的效率得到最大发挥,并得到了较大尺寸的木瓜蛋白酶晶体;当膜面积不同时,溶剂跨膜通量的衰减随着膜面积减小而趋于平缓.
채용취편불을희중공섬유미공막,고찰결정과정중단백질용액화세탈액류속적개변이급불동막면적대용제과막통량급목과단백매정체척촌적영향.결과표명:재이pH치위4.7적0.1 mol/L작산납위완충용액、1%적(NH_4)_3PO_4위침정제이급25%적MgCl_2용액작위세탈액적목과단백매동태막결정중,당유효막면적위62.83 cm~2,단백질용액화세탈액류속분별공제재4 744 μm/s화1.37×10~4μm/s적고류속시막조건적효솔득도최대발휘,병득도료교대척촌적목과단백매정체;당막면적불동시,용제과막통량적쇠감수착막면적감소이추우평완.
The PVDF hollow fiber micro-porous membranes were employed to study the influences of such fac-tors as flow velocities of protein solutions and stripping agent, membrane area on the process of membrane crys-tallization.It was found that, with 0.1 mol/L NaAc (pH= 4.7) as the buffer solution, 1% (NH_4)_3PO_4 as pre-cipitant and 25% MgCl_2 as stripping agent, when the membrane area was 62.83 cm~2, the flow velocity of pro-tein solutions was 4 744 μm/s and stripping agent was 1.37 × 10~4 μm/s, the largest size papain crystals were produced and the efficiency of the membrane was maximized.At the same time, the decrease of trans-mem-brane flux was tending to smooth with the reduction of the membrane area.