农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
1期
48-50
,共3页
宋向东%张之鑫%王巍巍%富健
宋嚮東%張之鑫%王巍巍%富健
송향동%장지흠%왕외외%부건
栽培大豆%异黄酮含量%测定%蛋白质含量%脂肪含量
栽培大豆%異黃酮含量%測定%蛋白質含量%脂肪含量
재배대두%이황동함량%측정%단백질함량%지방함량
Cultivated soybean%Isoflavone content%Determination%Protein content%Fat content
[目的]建立一种采用高效液相色谱测定大豆籽粒中异黄酮含量的方法,并对大豆异黄酮含量与蛋白质和脂肪含量进行相关性分析.[方法]样品先用浓度80%甲醇提取,而后100 ℃水解;色谱分离使用反相C18分析柱二元高压梯度洗脱(分析时间25 min),柱温40℃;使用二极管阵列检测器检测,波长为260 nm;大豆异黄酮含量与蛋白质和脂肪含量的相天性采用Origin6.0数据处理系统进行分析.[结果]经方法学验证建立的大豆异黄酮高效液相色谱方法准确、可靠,各异黄酮异构体的回收率在96.33%～104.20%范围内,RSD为2.56%～4.51%;通过对85份吉林省栽培大豆品系的异黄酬含量测定,初步明确了吉林省栽培大豆品系异黄酬含量的特点及范围,大豆异黄酮含量变幅为2.29～4.89 mg/g,平均含量为3.36 mg/g,含量超过4 mg/g的大豆品系5份,占所有测定总数的5.88%;大豆异黄酮含量与蛋白质含量呈显著的负相关;大豆异黄酮含量与脂肪含量呈不显著的正相关.[结论]吉林省栽培大豆品系异黄酮含量较高,培育高异黄酮含量和高脂肪含量的大豆品种是可行的.
[目的]建立一種採用高效液相色譜測定大豆籽粒中異黃酮含量的方法,併對大豆異黃酮含量與蛋白質和脂肪含量進行相關性分析.[方法]樣品先用濃度80%甲醇提取,而後100 ℃水解;色譜分離使用反相C18分析柱二元高壓梯度洗脫(分析時間25 min),柱溫40℃;使用二極管陣列檢測器檢測,波長為260 nm;大豆異黃酮含量與蛋白質和脂肪含量的相天性採用Origin6.0數據處理繫統進行分析.[結果]經方法學驗證建立的大豆異黃酮高效液相色譜方法準確、可靠,各異黃酮異構體的迴收率在96.33%～104.20%範圍內,RSD為2.56%～4.51%;通過對85份吉林省栽培大豆品繫的異黃酬含量測定,初步明確瞭吉林省栽培大豆品繫異黃酬含量的特點及範圍,大豆異黃酮含量變幅為2.29～4.89 mg/g,平均含量為3.36 mg/g,含量超過4 mg/g的大豆品繫5份,佔所有測定總數的5.88%;大豆異黃酮含量與蛋白質含量呈顯著的負相關;大豆異黃酮含量與脂肪含量呈不顯著的正相關.[結論]吉林省栽培大豆品繫異黃酮含量較高,培育高異黃酮含量和高脂肪含量的大豆品種是可行的.
[목적]건립일충채용고효액상색보측정대두자립중이황동함량적방법,병대대두이황동함량여단백질화지방함량진행상관성분석.[방법]양품선용농도80%갑순제취,이후100 ℃수해;색보분리사용반상C18분석주이원고압제도세탈(분석시간25 min),주온40℃;사용이겁관진렬검측기검측,파장위260 nm;대두이황동함량여단백질화지방함량적상천성채용Origin6.0수거처리계통진행분석.[결과]경방법학험증건립적대두이황동고효액상색보방법준학、가고,각이황동이구체적회수솔재96.33%～104.20%범위내,RSD위2.56%～4.51%;통과대85빈길림성재배대두품계적이황수함량측정,초보명학료길림성재배대두품계이황수함량적특점급범위,대두이황동함량변폭위2.29～4.89 mg/g,평균함량위3.36 mg/g,함량초과4 mg/g적대두품계5빈,점소유측정총수적5.88%;대두이황동함량여단백질함량정현저적부상관;대두이황동함량여지방함량정불현저적정상관.[결론]길림성재배대두품계이황동함량교고,배육고이황동함량화고지방함량적대두품충시가행적.
[Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat content,[Method]The isoflavonas were firstly extracted by 80%methanol and then hydrolyzed at 100℃.The chromatographic separation adopted a reversed-phase C18 analytical column with binary highpressure gradient elution, while its analysis time was 25 min and column temperature was 40℃.The diode array detector was used for monitoring with wavelength of 260 nm.The correlation between isofalvone content and protein or fat content was analyzed by data processing system Origin 6.0.[Result]The high performance liquid chromatograph for determination of isoflavones from soybean was verified to be accurate and reliable by methodology.The isoflavones of 85 soybean lines cultivated in Jilin Province were determined, and the results primarily showed the characters and ranges of isoflavones from soybean lines cultivated in Jilin Province, while the isoflavone content of soybeans ranged from 2.29 to 4.89 mg/g, and the average content was 3.36 mg/g.The isoflavone content of 5 soybean lines exceeded 4 mg/g, while there was a remarkably negative correlation between isoflavone content and protein content, and there was no significant positive correlation between isoflavone content and fat content.[Conclusion]The isoflavone content of soybean lines cultivated in Jilin Province is higher, so it is feasible for breeding the soybean lines with high isoflavone content and fat contetnt.
